Novel tools to study cell-ECM interactions, cell adhesion dynamics and migration

IF 6 2区 生物学 Q1 CELL BIOLOGY
Michal Dibus , Omkar Joshi , Johanna Ivaska
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引用次数: 0

Abstract

Integrin-mediated cell adhesion is essential for cell migration, mechanotransduction and tissue integrity. In vivo, these processes are regulated by complex physicochemical signals from the extracellular matrix (ECM). These nuanced cues, including molecular composition, rigidity and topology, call for sophisticated systems to faithfully explore cell behaviour. Here, we discuss recent methodological advances in cell-ECM adhesion research and compile a toolbox of techniques that we expect to shape this field in future. We outline methodological breakthroughs facilitating the transition from rigid 2D substrates to more complex and dynamic 3D systems, as well as advances in super-resolution imaging for an in-depth understanding of adhesion nanostructure. Selected methods are exemplified with relevant biological findings to underscore their applicability in cell adhesion research. We expect this new “toolbox” of methods will allow for a closer approximation of in vitro experimental setups to in vivo conditions, providing deeper insights into physiological and pathophysiological processes associated with cell-ECM adhesion.

研究细胞-ECM 相互作用、细胞粘附动力学和迁移的新工具
整合素介导的细胞粘附对于细胞迁移、机械传导和组织完整性至关重要。在体内,这些过程受到来自细胞外基质(ECM)的复杂物理化学信号的调控。这些微妙的线索包括分子组成、硬度和拓扑结构,需要复杂的系统来忠实地探索细胞行为。在此,我们将讨论细胞-ECM 粘附研究在方法上的最新进展,并整理出一个技术工具箱,希望它能在未来塑造这一领域。我们概述了促进从刚性二维基底向更复杂、更动态的三维系统过渡的方法学突破,以及深入了解粘附纳米结构的超分辨率成像的进展。所选方法将以相关生物学发现为例,强调其在细胞粘附研究中的适用性。我们希望这一新的 "工具箱 "方法能使体外实验设置更接近体内条件,从而更深入地了解与细胞-ECM 粘附相关的生理和病理生理过程。
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来源期刊
Current Opinion in Cell Biology
Current Opinion in Cell Biology 生物-细胞生物学
CiteScore
14.60
自引率
1.30%
发文量
79
审稿时长
93 days
期刊介绍: Current Opinion in Cell Biology (COCEBI) is a highly respected journal that specializes in publishing authoritative, comprehensive, and systematic reviews in the field of cell biology. The journal's primary aim is to provide a clear and readable synthesis of the latest advances in cell biology, helping specialists stay current with the rapidly evolving field. Expert authors contribute to the journal by annotating and highlighting the most significant papers from the extensive body of research published annually, offering valuable insights and saving time for readers by distilling key findings. COCEBI is part of the Current Opinion and Research (CO+RE) suite of journals, which leverages the legacy of editorial excellence, high impact, and global reach to ensure that the journal is a widely read resource integral to scientists' workflow. It is published by Elsevier, a publisher known for its commitment to excellence in scientific publishing and the communication of reproducible biomedical research aimed at improving human health. The journal's content is designed to be an invaluable resource for a diverse audience, including researchers, lecturers, teachers, professionals, policymakers, and students.
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