Sensitivity of the airway smooth muscle in terms of force, shortening and stiffness

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-09 DOI:10.1016/j.resp.2024.104264

Louis Gélinas, Andrés Rojas-Ruiz, Magali Boucher, Cyndi Henry, Ynuk Bossé

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引用次数: 0

Abstract

Eight pig tracheal strips were stimulated to contract with log increments of methacholine from 10^-8 to 10^-5 M. For each strip, the concentration-response was repeated four times in a randomized order to measure isometric force, isotonic shortening against a load corresponding to either 5 or 10 % of a reference force, and average force, stiffness, elastance and resistance over one cycle while the strip length was oscillating sinusoidally by 5 % at 0.2 Hz. For each readout, the logEC50 was calculated and compared. Isotonic shortening with a 5 % load had the lowest logEC50 (-7.13), yielding a greater sensitivity than any other contractile readout (p<0.05). It was followed by isotonic shortening with a 10 % load (-6.66), elastance (-6.46), stiffness (-6.46), resistance (-6.38), isometric force (-6.32), and average force (-6.30). Some of these differences were significant. For example, the EC50 with the average force was 44 % greater than with the elastance (p=0.001). The methacholine sensitivity is thus affected by the contractile readout being measured.

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气道平滑肌在力量、缩短和硬度方面的敏感性

对每个气管条，按随机顺序重复四次浓度-反应，以测量等长力、与相当于参考力 5% 或 10% 的负荷相对应的等张力缩短，以及一个周期内的平均力、硬度、弹性和阻力，同时气管条长度以 0.2 Hz 的频率正弦摆动 5%。对于每个读数，都要计算并比较 logEC50。负载为 5% 的等张缩短的对数EC50 最低（-7.13），其灵敏度高于任何其他收缩读数（p<0.05）。其次是 10% 负荷下的等张缩短（-6.66）、弹性（-6.46）、硬度（-6.46）、阻力（-6.38）、等长力（-6.32）和平均力（-6.30）。其中一些差异非常明显。例如，平均力的 EC50 比弹力的 EC50 高 44 %（P=0.001）。因此，甲氧胆碱的敏感性受到所测量的收缩读数的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.