Formulation and evaluation of azithromycin-loaded silver nanoparticles for the treatment of infected wounds

IF 5.2 2区 医学 Q1 PHARMACOLOGY & PHARMACY
Mohammed S. Saddik , Mostafa F. Al-Hakkani , Ahmed M. Abu-Dief , Mohamed S. Mohamed , Islam A. Al-Fattah , Mahmoud Makki , Mohamed A. El-Mokhtar , Marwa A. Sabet , M.S. Amin , Hoda A. Ahmed , Khalaf Al-Ghamdi , Mostafa K. Mohammad , Mohammad H.A. Hassan
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Abstract

Infected wounds pose a significant challenge in healthcare, requiring innovative therapeutic strategies. Therefore, there is a critical need for innovative pharmaceutical materials to improve wound healing and combat bacterial growth. This study examined the efficacy of azithromycin-loaded silver nanoparticles (AZM-AgNPs) in treating infected wounds. AgNPs synthesized using a green method with Quinoa seed extract were loaded with AZM. Characterization techniques, including X-ray Powder Diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), and Uv-Vis analysis were utilized. The agar diffusion assay and determination of the MIC were used to assess the initial antibacterial impact of the formulations on both MRSA and E. coli. In addition, the antimicrobial, wound-healing effects and histological changes following treatment with the AZM-AgNPs were assessed using an infected rat model. The nanoparticles had size of 24.9 ± 15.2 nm for AgNPs and 34.7 ± 9.7 nm for AZM-AgNPs. The Langmuir model accurately characterized the adsorption of AZM onto the AgNP surface, indicating a maximum loading capacity of 162.73 mg/g. AZM-AgNPs exhibited superior antibacterial properties in vivo and in vitro compared to controls. Using the agar diffusion technique, AZM-AgNPs showed enhanced zones of inhibition against E. coli and MRSA, which was coupled with decreased MIC levels. In addition, in vivo studies showed that AZM-AgNP treated rats had the best outcome characterized by improved healing process, lower bacterial counts and superior epithelialization, compared to the control group. In conclusion, AZM-AgNPs can be synthesized using a green method with Quinoa seed with successful loading of azithromycin onto silver nanoparticles. In vitro and in vivo studies suggest the promising use of AZM-AgNPs as an effective therapeutic agent for infected wounds.

Abstract Image

用于治疗感染性伤口的阿奇霉素负载银纳米粒子的制备与评估
感染性伤口是医疗保健领域的一大挑战,需要创新的治疗策略。因此,亟需创新的药物材料来改善伤口愈合和抑制细菌生长。本研究考察了阿奇霉素载银纳米粒子(AZM-AgNPs)治疗感染伤口的功效。银纳米粒子采用藜麦种子提取物的绿色方法合成,并载入了 AZM。表征技术包括 X 射线粉末衍射(XRD)、扫描电子显微镜(SEM)、透射电子显微镜(TEM)和紫外可见光分析。琼脂扩散试验和 MIC 测定用于评估制剂对 MRSA 和大肠杆菌的初步抗菌效果。此外,还利用感染大鼠模型评估了 AZM-AgNPs 处理后的抗菌、伤口愈合效果和组织学变化。AgNPs 纳米粒子的尺寸为 24.9 ± 15.2 nm,AZM-AgNPs 纳米粒子的尺寸为 34.7 ± 9.7 nm。Langmuir 模型准确地描述了 AZM 在 AgNP 表面的吸附情况,表明其最大负载能力为 162.73 mg/g。与对照组相比,AZM-AgNPs 在体内和体外均表现出卓越的抗菌特性。利用琼脂扩散技术,AZM-AgNPs 对大肠杆菌和 MRSA 的抑制区增大,同时 MIC 水平降低。此外,体内研究表明,与对照组相比,AZM-AgNP 治疗大鼠的疗效最好,其特点是愈合过程得到改善,细菌数量减少,上皮化程度提高。总之,AZM-AgNPs 可以用一种绿色方法与 Quinoa 种子合成,并成功地将阿奇霉素负载到银纳米粒子上。体外和体内研究表明,AZM-AgNPs 可作为治疗感染伤口的有效药物。
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来源期刊
International Journal of Pharmaceutics: X
International Journal of Pharmaceutics: X Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
6.60
自引率
0.00%
发文量
32
审稿时长
24 days
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