Molecular architecture of the actin cytoskeleton: From single cells to whole organisms using cryo-electron tomography

IF 6 2区 生物学 Q1 CELL BIOLOGY
Jonathan Schneider , Marion Jasnin
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引用次数: 0

Abstract

Cryo-electron tomography (cryo-ET) has begun to provide intricate views of cellular architecture at unprecedented resolutions. Considerable efforts are being made to further optimize and automate the cryo-ET workflow, from sample preparation to data acquisition and analysis, to enable visual proteomics inside of cells. Here, we will discuss the latest advances in cryo-ET that go hand in hand with their application to the actin cytoskeleton. The development of deep learning tools for automated annotation of tomographic reconstructions and the serial lift-out sample preparation procedure will soon make it possible to perform high-resolution structural biology in a whole new range of samples, from multicellular organisms to organoids and tissues.

肌动蛋白细胞骨架的分子结构:利用低温电子断层扫描技术从单细胞到整个生物体
低温电子断层扫描(cryo-ET)已开始以前所未有的分辨率提供细胞结构的复杂视图。为了实现细胞内可视化蛋白质组学,从样品制备到数据采集和分析的低温电子断层成像工作流程正在进一步优化和自动化。在此,我们将讨论低温电子显微镜的最新进展及其在肌动蛋白细胞骨架中的应用。用于断层重构自动注释的深度学习工具的开发,以及串行抬出样品制备程序,将很快使在从多细胞生物体到有机体和组织等全新范围的样品中进行高分辨率结构生物学研究成为可能。
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来源期刊
Current Opinion in Cell Biology
Current Opinion in Cell Biology 生物-细胞生物学
CiteScore
14.60
自引率
1.30%
发文量
79
审稿时长
93 days
期刊介绍: Current Opinion in Cell Biology (COCEBI) is a highly respected journal that specializes in publishing authoritative, comprehensive, and systematic reviews in the field of cell biology. The journal's primary aim is to provide a clear and readable synthesis of the latest advances in cell biology, helping specialists stay current with the rapidly evolving field. Expert authors contribute to the journal by annotating and highlighting the most significant papers from the extensive body of research published annually, offering valuable insights and saving time for readers by distilling key findings. COCEBI is part of the Current Opinion and Research (CO+RE) suite of journals, which leverages the legacy of editorial excellence, high impact, and global reach to ensure that the journal is a widely read resource integral to scientists' workflow. It is published by Elsevier, a publisher known for its commitment to excellence in scientific publishing and the communication of reproducible biomedical research aimed at improving human health. The journal's content is designed to be an invaluable resource for a diverse audience, including researchers, lecturers, teachers, professionals, policymakers, and students.
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