The immune landscape and prognostic analysis of CXCL8 immune-related genes in cervical squamous cell carcinoma

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-04-10 DOI:10.1002/tox.24283

Xiaoqian Zhang, Jian Yang, Qianqian Feng, Liping Gu, Gongzhao Qin, Chen Cheng, Shunyu Hou, Zhouhong Shi

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引用次数: 0

Abstract

Cervical squamous cell carcinoma (CESC), one of the most common malignancies in women, imposes a significant burden on women's health worldwide. Despite extensive research, the molecular and pathogenic mechanisms of cervical squamous cell carcinoma and CESC remain unclear. This study aimed to explore the immune-related genes, immune microenvironment infiltration, and prognosis of CESC, providing a theoretical basis for guiding clinical treatment. Initially, by mining four gene sets and immune-related gene sets from public databases, 14 immune-related genes associated with CESC were identified. Through univariate and multivariate COX regression analyses, as well as lasso regression analysis, four CESC-independent prognostic genes were identified, and a prognostic model was constructed, dividing them into high and low-risk groups. The correlation between these genes and immune cells and immune functions were explored through ssGSEA enrichment analysis, revealing a close association between the high-risk group and processes such as angiogenesis and epithelial–mesenchymal transition. Furthermore, using public databases and qRT-PCR experiments, significant differences in CXCL8 expression between normal cervical cells and cervical cancer cells were discovered. Subsequently, a CXCL8 knockdown plasmid was constructed, and the efficiency of CXCL8 knockdown was validated in two CESC cell lines, MEG-01 and HCE-1. Through CCK-8, scratch, and Transwell assays, it was confirmed that CXCL8 knockdown could inhibit the proliferation, invasion, and migration abilities of CESC cells. Targeting CXCL8 holds promise for personalized therapy for CESC, providing a strong theoretical basis for achieving clinical translation.

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宫颈鳞状细胞癌中 CXCL8 免疫相关基因的免疫图谱和预后分析

宫颈鳞状细胞癌（CESC）是女性最常见的恶性肿瘤之一，给全世界妇女的健康带来了沉重负担。尽管进行了大量研究，但宫颈鳞状细胞癌和 CESC 的分子和致病机制仍不清楚。本研究旨在探讨CESC的免疫相关基因、免疫微环境浸润和预后，为指导临床治疗提供理论依据。首先，通过挖掘公共数据库中的四个基因组和免疫相关基因组，确定了14个与CESC相关的免疫相关基因。通过单变量和多变量COX回归分析以及lasso回归分析，确定了4个与CESC无关的预后基因，并构建了预后模型，将其分为高危组和低危组。通过ssGSEA富集分析探讨了这些基因与免疫细胞和免疫功能的相关性，发现高风险组与血管生成和上皮-间质转化等过程密切相关。此外，利用公共数据库和 qRT-PCR 实验发现，正常宫颈细胞和宫颈癌细胞的 CXCL8 表达存在显著差异。随后，研究人员构建了CXCL8基因敲除质粒，并在MEG-01和HCE-1两种CESC细胞系中验证了CXCL8基因敲除的效率。通过CCK-8、划痕和Transwell试验证实，CXCL8敲除可抑制CESC细胞的增殖、侵袭和迁移能力。以CXCL8为靶点有望用于CESC的个性化治疗，为实现临床转化提供了坚实的理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.