{"title":"The distinctive short-term response of late-pregnant prolific ewes to various doses and mixtures of propylene glycol and glycerol drenching","authors":"Tamir Alon , Alexander Rosov , Lilya Lifshitz , Uzi Moallem","doi":"10.1016/j.anifeedsci.2024.115957","DOIUrl":null,"url":null,"abstract":"<div><p>Pregnancy toxemia (<strong>PT</strong>) is the most frequent metabolic disorder for ewes in late pregnancy. Although propylene glycol (<strong>PG</strong>) and glycerol (<strong>GLY</strong>) are common glycogenic supplements for treating PT in ewes, the relative benefit of these supplements is not clear. In a previous study, it was demonstrated that PG was mainly effective in reducing the β-hydroxybutyrate acid (BHB) concentration, whereas GLY was more effective in increasing the plasma glucose concentration. Therefore, the objectives of the present study were to determine the effects of drenching various doses and combinations of PG and GLY to late-pregnant ewes in order to obtain a simultaneous increase in glucose and a decrease in BHB. Sixty-six multiparous Afec-Assaf ewes bearing at least two fetuses were used in 3 consecutive experiments. In all experiments, blood samples were collected twice before drenching (at 0630 and 0645 h), then the ewes were drenched with each supplement at 0700 h, and blood samples were taken every hour for 12–14 h post-drenching. In experiment 1, ewes were drenched either with 1) Control <strong>(CTL1,</strong> n = 6<strong>)</strong> – 55 mL water, 2) <strong>PG100</strong> (n = 6) – 106 mL of PG, 3) <strong>GLY100</strong> (n = 6) –108 mL of Koforin (containing 80 % glycerol, 15 % water, and 5 % ash), 4) <strong>PG50</strong> (n = 6) – 53 mL of PG, and 5) <strong>GLY50</strong> (n = 6) – 54 mL of Koforin. The plasma glucose concentration was higher after the GLY50 (<em>P</em> = 0.03) and GLY100 (<em>P</em> = 0.01) treatments than in CTL1. The plasma BHB concentration was lower after the PG50 (<em>P</em> = 0.001) and GLY50 (<em>P</em> = 0.02) treatments and tended to be lower in the PG100-treated ewes (<em>P</em> = 0.10) than in the CTL1 ewes. In experiment 2, ewes were drenched with 1) Control (<strong>CTL2,</strong> n = 6<strong>)</strong> –55 mL water, 2) <strong>MIX50</strong> (n = 6) –26.5 mL PG + 27 mL Koforin, and 3) <strong>MIX100</strong> (n = 6) –53 mL PG + 54 mL Koforin. No differences were observed among treatments for the main blood metabolites. In experiment 3, ewes were drenched with 1) Control <strong>(CTL3,</strong> n = 6<strong>)</strong> – 110 mL water, 2) <strong>MIX200(1:1)</strong> (n = 6) –106 mL PG +108 mL Koforin, and 3) <strong>MIX200(3:7)</strong> (n = 6) – 63.4 mL PG +150.6 mL Koforin. The plasma glucose concentrations were 17.8 % and 20.9 %, respectively, higher in the MIX200(1:1) and MIX200(3:7) ewes than in the CTL3 ewes (<em>P</em> < 0.01). The plasma BHB concentrations in the MIX200(1:1) and MIX200(3:7) ewes were 42 % and 47 %, respectively, lower than in the CTL3 ewes (<em>P</em> < 0.03). The NEFA concentration in the MIX200(1:1) and MIX200(3:7) ewes was 60 % and 57 %, respectively, lower than in the CTL3 ewes (<em>P</em> = 0.008). In conclusion, MIX200(1:1) and MIX200(3:7) were the most effective approaches to increasing glucose and reducing the BHB and NEFA concentrations concomitantly in the plasma of late-pregnant ewes.</p></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Feed Science and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0377840124000853","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
Abstract
Pregnancy toxemia (PT) is the most frequent metabolic disorder for ewes in late pregnancy. Although propylene glycol (PG) and glycerol (GLY) are common glycogenic supplements for treating PT in ewes, the relative benefit of these supplements is not clear. In a previous study, it was demonstrated that PG was mainly effective in reducing the β-hydroxybutyrate acid (BHB) concentration, whereas GLY was more effective in increasing the plasma glucose concentration. Therefore, the objectives of the present study were to determine the effects of drenching various doses and combinations of PG and GLY to late-pregnant ewes in order to obtain a simultaneous increase in glucose and a decrease in BHB. Sixty-six multiparous Afec-Assaf ewes bearing at least two fetuses were used in 3 consecutive experiments. In all experiments, blood samples were collected twice before drenching (at 0630 and 0645 h), then the ewes were drenched with each supplement at 0700 h, and blood samples were taken every hour for 12–14 h post-drenching. In experiment 1, ewes were drenched either with 1) Control (CTL1, n = 6) – 55 mL water, 2) PG100 (n = 6) – 106 mL of PG, 3) GLY100 (n = 6) –108 mL of Koforin (containing 80 % glycerol, 15 % water, and 5 % ash), 4) PG50 (n = 6) – 53 mL of PG, and 5) GLY50 (n = 6) – 54 mL of Koforin. The plasma glucose concentration was higher after the GLY50 (P = 0.03) and GLY100 (P = 0.01) treatments than in CTL1. The plasma BHB concentration was lower after the PG50 (P = 0.001) and GLY50 (P = 0.02) treatments and tended to be lower in the PG100-treated ewes (P = 0.10) than in the CTL1 ewes. In experiment 2, ewes were drenched with 1) Control (CTL2, n = 6) –55 mL water, 2) MIX50 (n = 6) –26.5 mL PG + 27 mL Koforin, and 3) MIX100 (n = 6) –53 mL PG + 54 mL Koforin. No differences were observed among treatments for the main blood metabolites. In experiment 3, ewes were drenched with 1) Control (CTL3, n = 6) – 110 mL water, 2) MIX200(1:1) (n = 6) –106 mL PG +108 mL Koforin, and 3) MIX200(3:7) (n = 6) – 63.4 mL PG +150.6 mL Koforin. The plasma glucose concentrations were 17.8 % and 20.9 %, respectively, higher in the MIX200(1:1) and MIX200(3:7) ewes than in the CTL3 ewes (P < 0.01). The plasma BHB concentrations in the MIX200(1:1) and MIX200(3:7) ewes were 42 % and 47 %, respectively, lower than in the CTL3 ewes (P < 0.03). The NEFA concentration in the MIX200(1:1) and MIX200(3:7) ewes was 60 % and 57 %, respectively, lower than in the CTL3 ewes (P = 0.008). In conclusion, MIX200(1:1) and MIX200(3:7) were the most effective approaches to increasing glucose and reducing the BHB and NEFA concentrations concomitantly in the plasma of late-pregnant ewes.
期刊介绍:
Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding.
Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome.
The journal covers the following areas:
Nutritive value of feeds (e.g., assessment, improvement)
Methods of conserving and processing feeds that affect their nutritional value
Agronomic and climatic factors influencing the nutritive value of feeds
Utilization of feeds and the improvement of such
Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins)
Mathematical models relating directly to animal-feed interactions
Analytical and experimental methods for feed evaluation
Environmental impacts of feed technologies in animal production.