Molecular detection of Lotmaria passim in intestine of Japanese honeybees (Apis cerana japonica)

IF 0.7 4区农林科学 Q4 ENTOMOLOGY

Entomological Science Pub Date : 2024-04-09 DOI:10.1111/ens.12571

Akihiko Suzuki, Yoshiko Sakamoto

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Abstract

Crithidia mellificae and Lotmaria passim are trypanosomatids that infect honeybees, and many studies suggest that both parasites contribute to the decline in the honeybee population. The Japanese honeybee (Apis cerana japonica) is a native honeybee that inhabits various areas of Japan and is one of the most important pollinators. Both parasites have been well studied in the western honeybee (Apis mellifera) but have been poorly investigated in A. c. japonica. The present study investigated the presence of C. mellificae and L. passim in one feral and six managed A. c. japonica colonies at three different institutes. Five out of seven colonies were polymerase chain reaction-positive for L. passim (71.4%); however, C. mellificae was not detected. Four of the five colonies were positive in both the midgut and hindgut, but one was positive only in the hindgut. A homology search and phylogenetic tree of the glycosomal glyceraldehyde-3-phosphate dehydrogenase (gGAPDH) and cytochrome b (Cytb) genes obtained in this study indicated that the sequences belonged to L. passim. The results of this study emphasize concerns regarding the health of this ecologically important pollinator and the importance of further investigation of the prevalence of L. passim in A. c. japonica in a wider area of Japan.

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日本蜜蜂肠道中Lotmaria passim的分子检测

Crithidia mellificae 和 Lotmaria passim 是感染蜜蜂的锥虫，许多研究表明，这两种寄生虫导致了蜜蜂数量的减少。日本蜜蜂（Apis cerana japonica）是一种原生蜜蜂，栖息在日本多个地区，是最重要的授粉媒介之一。这两种寄生虫对西方蜜蜂（Apis mellifera）的研究较多，但对日本蜜蜂（Apis cerana japonica）的研究较少。本研究调查了三个不同研究所的一个野外蜂群和六个人工饲养的 A. c. japonica 蜂群中是否存在 C. mellificae 和 L. passim。7 个群落中有 5 个聚合酶链式反应阳性（71.4%），但未检测到 L. passim；5 个群落中有 4 个聚合酶链式反应阳性（71.4%），但未检测到 C. mellificae。五个菌落中有四个在中肠和后肠都呈阳性，但有一个只在后肠呈阳性。本研究中获得的糖体甘油醛-3-磷酸脱氢酶（gGAPDH）和细胞色素 b（Cytb）基因的同源性搜索和系统发生树表明，这些序列属于 L. passim。这项研究的结果强调了人们对这种具有重要生态意义的授粉昆虫健康状况的关注，以及在日本更广泛地区进一步调查 L. passim 在 A. c. japonica 中的流行情况的重要性。

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来源期刊

Entomological Science 生物-昆虫学

CiteScore

2.00

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Entomological Science is the official English language journal of the Entomological Society of Japan. The Journal publishes original research papers and reviews from any entomological discipline or from directly allied field in ecology, behavioral biology, physiology, biochemistry, development, genetics, systematics, morphology, evolution and general entomology. Papers of applied entomology will be considered for publication if they significantly advance in the field of entomological science in the opinion of the Editors and Editorial Board.