Stable isotope insights into arthropod food chains and nitrogen cycling in a rehabilitated tailings chronosequence

Abstract

Field studies in post-mining sites on epigeic invertebrate groups are scarce despite their importance in pedogenesis and ecosystem multifunctionality. This research investigated the diversity, abundance and succession of aboveground invertebrates in a rehabilitated Pb/Zn tailings chronosequence of 5, 20 and 35 years. The study also explored the trophic relationship of selected arthropods and characterized the nitrogen (N) cycle using stable isotope measurements. The abundance and species richness in most investigated groups and the dominance index Berger–Parker (BP) of aboveground invertebrates increased with rehabilitation age from 0.17 BP in early to 0.31 BP in late stage. Elemental and stable isotope ratio analysis showed that N and C soil content increased and the C/N ratio decreased with age, yet despite this increased N availability in the system, the maturing N-cycle used N efficiently. The lack of large N losses from the system despite substantial atmospheric deposition inputs was indicated by the fact that N isotope ratios (δ¹⁵N) in plants and animals became significantly more negative with rehabilitation age, −6.0 δ¹⁵N for plants, −5.0 δ¹⁵N for herbivores and 3.0 δ¹⁵N for carnivores. The length of the invertebrate food chain expanded by more than half a trophic level (2.7‰ δ¹⁵N) for top predator Coleoptera from early to late stage, probably reflecting more complex food webs including intra-guild predation in older communities. In conclusion, δ¹⁵N measurements in plants and animals provided novel insights into the N-cycle, accumulative N flows and the trophic position in post-mining sites. It is proposed that isotope ratio measurements could be used as easy-to-measure, integrating indicators of nutrient cycling and the soil food web complexity of rehabilitated mine tailings and similar soil ecosystems.