A comprehensive study on chemical and biological profiles of Algerian Azadirachta indica (A. Juss)

IF 1.3 4区生物学 Q4 CHEMISTRY, MEDICINAL

Phytochemistry Letters Pub Date : 2024-04-08 DOI:10.1016/j.phytol.2024.03.017

Ahmed Boukeloua , Soudani Leila , Hamdi Bendif , Ahmed Mustafa Elkadeem , Mehmet Boga , Maria Luísa Serralheiro , Mustafa Abdullah Yilmaz , Ismail Yener , Stefania Garzoli

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Abstract

The study aimed to analyse the chemical and biological composition of Azadirachta indica (A. Juss) (A. indica) leaves. Minerals were quantified using inductively coupled plasma-mass spectrometry (ICP-MS). Phenolic compounds and water-soluble vitamins were identified via LC-MS/MS. Antioxidant activity was assessed using ABTS cation radicals and cupric reducing antioxidant capacity (CUPRAC), while anticholinesterase effect was tested against butyrylcholinesterase (BChE) and Acetylcholinesterase (AChE). Results revealed significant levels of major elements (e.g., Zn, Cu) making it a promising source. Predominant phenolic acids included tannic and salicylic acid, with major flavonoids being hyperoside, kaempferol, and quercetin. Main water-soluble vitamins were nicotinic acid, nicotinamide, and pantothenic acid, followed by pyridoxine, thiamine, and riboflavin. Ethanol extract exhibited moderate antioxidant activity (30.02 % inhibition of ABTS radicals; IC50 0.918 μg/mL for CUPRAC and low inhibition of BChE and AChE (2.29 % and 3.17 % respectively). These findings highlight A. indica as a valuable natural source of nutritionally significant compounds and antioxidants.

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关于阿尔及利亚 Azadirachta indica（A. Juss）化学和生物特征的综合研究

该研究旨在分析 Azadirachta indica (A. Juss) (A. indica) 叶子的化学和生物成分。使用电感耦合等离子体质谱法（ICP-MS）对矿物质进行了定量。酚类化合物和水溶性维生素通过 LC-MS/MS 进行鉴定。抗氧化活性采用 ABTS 阳离子自由基和铜还原抗氧化能力（CUPRAC）进行评估，抗胆碱酯酶作用则采用丁酰胆碱酯酶（BChE）和乙酰胆碱酯酶（AChE）进行检测。结果表明，主要元素（如锌、铜）的含量很高，使其成为一种很有前景的来源。主要酚酸包括单宁酸和水杨酸，主要类黄酮包括金丝桃苷、山柰酚和槲皮素。主要的水溶性维生素是烟酸、烟酰胺和泛酸，其次是吡哆醇、硫胺素和核黄素。乙醇提取物具有中等抗氧化活性（对 ABTS 自由基的抑制率为 30.02%；CUPRAC 的 IC50 为 0.918 μg/mL；对 BChE 和 AChE 的抑制率较低（分别为 2.29% 和 3.17%）。这些发现凸显了籼稻是具有重要营养价值的化合物和抗氧化剂的宝贵天然来源。

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来源期刊

Phytochemistry Letters 生物-生化与分子生物学

CiteScore

3.00

自引率

11.80%

发文量

190

审稿时长

34 days

期刊介绍： Phytochemistry Letters invites rapid communications on all aspects of natural product research including: • Structural elucidation of natural products • Analytical evaluation of herbal medicines • Clinical efficacy, safety and pharmacovigilance of herbal medicines • Natural product biosynthesis • Natural product synthesis and chemical modification • Natural product metabolism • Chemical ecology • Biotechnology • Bioassay-guided isolation • Pharmacognosy • Pharmacology of natural products • Metabolomics • Ethnobotany and traditional usage • Genetics of natural products Manuscripts that detail the isolation of just one new compound are not substantial enough to be sent out of review and are out of scope. Furthermore, where pharmacology has been performed on one new compound to increase the amount of novel data, the pharmacology must be substantial and/or related to the medicinal use of the producing organism.