Development of a microfluidic-assisted open-source 3D bioprinting system (MOS3S) for the engineering of hierarchical tissues

IF 2 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC

HardwareX Pub Date : 2024-04-02 DOI:10.1016/j.ohx.2024.e00527

Sajad Mohammadi , Salvatore D’Alessandro , Fabiano Bini , Franco Marinozzi , Gianluca Cidonio

{"title":"Development of a microfluidic-assisted open-source 3D bioprinting system (MOS3S) for the engineering of hierarchical tissues","authors":"Sajad Mohammadi , Salvatore D’Alessandro , Fabiano Bini , Franco Marinozzi , Gianluca Cidonio","doi":"10.1016/j.ohx.2024.e00527","DOIUrl":null,"url":null,"abstract":"<div><p>The engineering of new 3D bioprinting approaches has shown great promise in the field of tissue engineering and disease modelling. However, the high cost of commercial 3D bioprinters has limited their accessibility, especially to those laboratories in resource-limited settings. Moreover, the need for a 3D bioprinting system capable of dispensing multiple materials is growing apace. Therefore, the development of a Microfluidic-assisted Open Source 3D bioprinting System (MOS3S) for the engineering of hierarchical tissues is needed to progress in fabricating functional tissues, but with a technology accessible to a wider range of researchers. The MOS3S platform is designed to allow the deposition of biomaterial inks using microfluidic printheads or coaxial nozzles for the <em>in-situ</em> crosslinking and scaffolds fabrication. The coupling of 3D printed syringe pumps with the motion control system is used for driving the tunable extrusion of inks for the fabrication of centimeter scale hierarchical lattice constructs for tissue engineering purposes. MOS3S performance have been validated to fabricate high-resolution structures with coaxial microfluidic technology, opening to new frontiers for seminal studies in pre-clinical disease modelling and tissue regeneration.</p></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"18 ","pages":"Article e00527"},"PeriodicalIF":2.0000,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S246806722400021X/pdfft?md5=446bbad68767afaeac425d2c0dfbea04&pid=1-s2.0-S246806722400021X-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"HardwareX","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S246806722400021X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}

引用次数: 0

Abstract

The engineering of new 3D bioprinting approaches has shown great promise in the field of tissue engineering and disease modelling. However, the high cost of commercial 3D bioprinters has limited their accessibility, especially to those laboratories in resource-limited settings. Moreover, the need for a 3D bioprinting system capable of dispensing multiple materials is growing apace. Therefore, the development of a Microfluidic-assisted Open Source 3D bioprinting System (MOS3S) for the engineering of hierarchical tissues is needed to progress in fabricating functional tissues, but with a technology accessible to a wider range of researchers. The MOS3S platform is designed to allow the deposition of biomaterial inks using microfluidic printheads or coaxial nozzles for the in-situ crosslinking and scaffolds fabrication. The coupling of 3D printed syringe pumps with the motion control system is used for driving the tunable extrusion of inks for the fabrication of centimeter scale hierarchical lattice constructs for tissue engineering purposes. MOS3S performance have been validated to fabricate high-resolution structures with coaxial microfluidic technology, opening to new frontiers for seminal studies in pre-clinical disease modelling and tissue regeneration.

Abstract Image

查看原文本刊更多论文

开发用于分层组织工程的微流体辅助开源三维生物打印系统（MOS3S）

新的三维生物打印工程方法在组织工程和疾病建模领域大有可为。然而，商用三维生物打印机的高昂成本限制了它们的普及，尤其是对那些资源有限的实验室而言。此外，对能够分配多种材料的三维生物打印系统的需求也在快速增长。因此，需要开发一种用于分层组织工程的微流体辅助开源三维生物打印系统（MOS3S），以便在制造功能性组织方面取得进展，同时让更多的研究人员能够使用这种技术。MOS3S 平台旨在利用微流体打印头或同轴喷嘴沉积生物材料墨水，进行原位交联和支架制造。三维打印注射泵与运动控制系统的耦合用于驱动墨水的可调挤出，以制造用于组织工程的厘米级分层晶格结构。MOS3S 的性能已得到验证，可利用同轴微流体技术制造高分辨率结构，为临床前疾病建模和组织再生方面的开创性研究开辟了新领域。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

HardwareX Engineering-Industrial and Manufacturing Engineering

CiteScore

4.10

自引率

18.20%

发文量

124

审稿时长

24 weeks

期刊介绍： HardwareX is an open access journal established to promote free and open source designing, building and customizing of scientific infrastructure (hardware). HardwareX aims to recognize researchers for the time and effort in developing scientific infrastructure while providing end-users with sufficient information to replicate and validate the advances presented. HardwareX is open to input from all scientific, technological and medical disciplines. Scientific infrastructure will be interpreted in the broadest sense. Including hardware modifications to existing infrastructure, sensors and tools that perform measurements and other functions outside of the traditional lab setting (such as wearables, air/water quality sensors, and low cost alternatives to existing tools), and the creation of wholly new tools for either standard or novel laboratory tasks. Authors are encouraged to submit hardware developments that address all aspects of science, not only the final measurement, for example, enhancements in sample preparation and handling, user safety, and quality control. The use of distributed digital manufacturing strategies (e.g. 3-D printing) is encouraged. All designs must be submitted under an open hardware license.