The effect of different concentrations of laminarin on the quality of cryopreserved ram semen.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2024-01-01 DOI:10.54680/fr24110110812

Nahid Zangishhi, Hadi Hajarian, Hamed Karamishabankareh, Leila Soltani

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引用次数: 0

Abstract

BACKGROUND Increasingly, sheep breeders are using artificial insemination to produce lambs, so finding methods that preserve ram sperm can be useful. OBJECTIVE To determine the protective effects of different concentrations of laminarin on ram sperm motility, viability, abnormalities, membrane, and DNA integrity, superoxide dismutase enzyme (SOD) activity, and malondialdehyde (MDA) production after freeze-thawing. MATERIALS AND METHODS The ejaculates of four rams were collected and stored at 35 degree C. Semen samples were diluted with a tris-base extender containing 100, 200, 400, and 800 ug/mL of laminarin and a control extender containing no laminarin, then frozen in liquid nitrogen after 4 h in the refrigerator. RESULTS In the treatment of frozen-thawed spermatozoa with 800 ug/mL laminarin, motility, viability, membrane integrity, and DNA integrity were significantly higher than in the control. In spermatozoa that were exposed to 800 ug/mL laminarin after thawing, MDA production was significantly lower than in the control group. The percentage of abnormal spermatozoa in 800 ug/mL laminarin was significantly lower than that in the control. CONCLUSION The addition of 800 ug/mL laminarin to the freezing extender increases motility, viability, SOD activity, and plasma membrane integrity, while reducing abnormality and MDA production in freeze-thawed ram semen. https://doi.org/10.54680/fr24110110812.

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不同浓度的层粘蛋白对冷冻保存的公羊精液质量的影响。

背景越来越多的绵羊饲养者使用人工授精来生产羔羊，因此找到能保存公羊精子的方法非常有用。目的确定不同浓度的层粘连素对冻融后公羊精子的活力、存活率、畸形、膜和 DNA 完整性、超氧化物歧化酶 (SOD) 活性以及丙二醛 (MDA) 生成的保护作用。用含有 100、200、400 和 800 微克/毫升层粘连素的三碱基扩展剂和不含层粘连素的对照扩展剂稀释精液样本，然后在冰箱中冷冻 4 小时后再放入液氮中冷冻。结果用 800 微克/毫升层粘连素处理冻融精子时，精子的运动能力、存活率、膜完整性和 DNA 完整性明显高于对照组。解冻后接触 800 微克/毫升层粘蛋白的精子中，MDA 的产生量明显低于对照组。结论在冷冻扩展剂中添加 800 微克/毫升的层黏蛋白可提高冻融公羊精液的活力、存活率、SOD 活性和质膜完整性，同时减少畸形精子和 MDA 的产生。https://doi.org/10.54680/fr24110110812。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.