Comparison of different freezing methods for micro-volume semen.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2024-01-01 DOI:10.54680/fr24110110412

Liu Ji, Yan-Hong Li, Yan-Hua Zhou, Xiao-Xiao Wang, Ling-Xi Tong, Hong-Hui Wang

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引用次数: 0

Abstract

BACKGROUND Mico-volume semen freezing is essential and used popularly for fertility preservation of patients suffering cancer or undergoing male reproductive system related surgeries, and for other reasons that may risk fertility potential in ART cycles. However, clinicians and embryologists still face some unresolved technical and theoretical issues about the frozen-thawed efficiency. OBJECTIVE To choose the appropriate freezing method for different volumes of normal semen samples. MATERIALS AND METHODS We investigated the frozen-thawed outcomes of semen with different volumes (20 uL, 50 uL, 100 uL, 200 uL, 500 uL and 1 mL) using two freezing methods (FLNV, static liquid nitrogen vapour cooling followed by liquid nitrogen preservation; RFLN, direct rapid freezing in liquid nitrogen) and analyzed the vitality, progressive motility and DNA fragmentation index of thawed sperm. RESULTS We found that semen freezing with volumes more than 100 uL had better outcomes than volumes less than or equal to 50 uL after thawing. FLNV presented a higher efficiency for cryopreservation of semen with volumes less than 50 uL. CONCLUSION For smaller (micro) volumes, the FLNV technique is better than the RFLN method. https://doi.org/10.54680/fr24110110412.

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微量精液不同冷冻方法的比较

背景微量精液冷冻对于癌症患者或接受男性生殖系统相关手术的患者的生育力保存以及其他可能危及 ART 循环中生育潜力的原因至关重要，并得到广泛应用。然而，临床医生和胚胎学家在冷冻解冻效率方面仍面临一些尚未解决的技术和理论问题。目的为不同容量的正常精液样本选择合适的冷冻方法。材料与方法我们研究了不同容量（20 uL、50 uL、100 uL、200 uL、500 uL 和 1 mL）精液的两种冷冻方法（FLNV，静态液氮蒸汽冷却后液氮保存；RFLN，直接在液氮中快速冷冻）的冻融结果，并分析了解冻精子的活力、进行性运动能力和 DNA 断裂指数。结果我们发现，解冻后精液量大于 100 uL 的冷冻效果优于小于或等于 50 uL 的冷冻效果。对于体积小于 50 uL 的精液，FLNV 的冷冻保存效率更高。结论对于体积较小（微量）的精液，FLNV 技术比 RFLN 方法更好。https://doi.org/10.54680/fr24110110412。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.