Suplementasi Ekstrak Spirulina sp. Pada Pakan Meningkatkan Toleransi Post-Larva L. vannamei Terhadap Stres Salinitas

IF 0.5 4区 地球科学 Q3 Earth and Planetary Sciences
Raisha Fahmida Dwi Lestari, Ervia Yudiati, Ali Djunaedi
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引用次数: 0

Abstract

Litopenaeus vannamei merupakan komoditas yang bernilai ekonomi tinggi pada sektor perikanan dan budidaya. Spirulina sp. merupakan mikroalga yang mengandung nutrisi tinggi dan senyawa potensial yang memiliki efek terapeutik. Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi ekstrak Spirulina sp. dalam pakan terhadap tingkat kelangsungan hidup, pertumbuhan, dan tingkat kelangsungan hidup L. vannamei pada uji stres salinitas. Hewan uji yang digunakan adalah post-larvae (PL 8) L. vannamei dengan bobot rata-rata 0,002 g/ekor. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) yang terdiri atas 3 konsentrasi, yaitu 3 mg/kg (SP 3), 6 mg/kg (SP 6), dan 9 mg/kg (SP 9) serta satu kontrol dengan masing-masing 4 pengulangan. L. vannamei dipelihara dan diberi pakan suplementasi Spirulina sp. selama 14 hari. Pada akhir penelitian dilakukan uji stres salinitas dengan menggunakan tabung falcon yang masing-masing diisi 20 ekor PL dan dipaparkan pada akuades (45 mL) dan ditutup rapat. PL diamati dan dihitung jumlah kelulushidupannya setiap 10 menit. Waktu pengamatan dihentikan ketika kematian udang mencapai 50%.Hasil penelitian menunjukkan bahwa pemberian suplementasi ekstrak Spirulina sp. pada pakan tidak berpengaruh nyata (p>0,05) terhadap pertumbuhan dan tingkat kelangsungan hidup udang. Namun, perlakuan SP 9 menunjukkan pengaruh nyata (p<0,05) terhadap tingkat kelangsungan pada uji stres salinitas. Suplementasi ekstrak Spirulina sp. mampu meningkatkan toleransi L. vannamei terhadap stres salinitas.  Litopenaeus vannamei is high economically viable cultivate in the aquaculture sectors. Spirulina sp. is high nutrients microalgae and potential compound that have therapeutic effects. This study aims to determine the effect of dietary supplementation of Spirulina sp. on feed towards survival rate, growth, and resistance of L. vannamei in the salinity stress test. This experiment was using post-larvae (PL 8) (0.002 g/ind). This study used a completely randomized design (CRD) consisting of 3 concentrations, namely 3 mg/kg (SP 3), 6 mg/kg (SP 6), and 9 mg/kg (SP 9), and one control with 4 repetitions for each treatment.  L. vannamei was reared and fed with Spirulina sp. supplementation for 14 days. On stress salinity test, PL were put in the falcon tube filled with aqua dest with 20 PL each and exposed until 50% mortality. The results showed that the supplementation of Spirulina sp. on feed had no significant effect (p>0.05) on the growth and survival rate of shrimp. However, the SP 9 treatment showed a significant effect (p<0.05) on the survival rate in the salinity stress test. Is concluded that supplementation of Spirulina sp. in the diet managed to increase the resistance of L. vannamei toward stress salinity.
在饲料中添加螺旋藻提取物可提高凡纳滨鲤后代对盐度胁迫的耐受性
凡纳滨对虾是渔业和水产养殖业中具有很高经济价值的商品。螺旋藻是一种微藻,含有高营养成分和具有治疗作用的潜在化合物。本研究旨在确定在饲料中添加螺旋藻提取物对凡纳滨鲤在盐度胁迫试验中的存活率、生长和存活率的影响。试验动物为凡纳滨鲤的后幼体(PL 8),平均体重为 0.002 克/头。该研究采用完全随机设计(CRD)方法,包括 3 种浓度,即 3 毫克/千克(SP 3)、6 毫克/千克(SP 6)和 9 毫克/千克(SP 9),以及一种对照,每种浓度重复 4 次。用螺旋藻补充剂饲养凡纳滨鲤 14 天。在研究结束时,使用猎鹰管进行盐度胁迫试验,每个猎鹰管中装入 20 个 PL,并将其置于蒸馏水(45 mL)中并紧闭。每隔 10 分钟观察并计算 PL 的存活数量。结果表明,在饲料中添加螺旋藻提取物对对虾的生长和存活率没有显著影响(p>0.05)。但 SP 9 处理对对虾的生长和存活率有显著影响(p0.05)。然而,在盐度胁迫试验中,SP 9 处理对虾的存活率有显著影响(p<0.05)。因此,在日粮中添加螺旋藻能提高凡纳滨对虾对盐胁迫的抵抗力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Marine Research
Journal of Marine Research 地学-海洋学
自引率
0.00%
发文量
1
审稿时长
3 months
期刊介绍: The Journal of Marine Research publishes peer-reviewed research articles covering a broad array of topics in physical, biological and chemical oceanography. Articles that deal with processes, as well as those that report significant observations, are welcome. In the area of biology, studies involving coupling between ecological and physical processes are preferred over those that report systematics. Authors benefit from thorough reviews of their manuscripts, where an attempt is made to maximize clarity. The time between submission and publication is kept to a minimum; there is no page charge.
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