Analisis Kandungan Klorofil -a dan Kepadatan Diatom Thalassiosira sp. Dengan Penggunaan Konsentrasi Silikat yang Berbeda

IF 0.5 4区地球科学 Q3 Earth and Planetary Sciences

Journal of Marine Research Pub Date : 2024-01-11 DOI:10.14710/jmr.v13i1.35303

Syahrial Varrel Cannavaro, Hadi Endrawati, W. A. Setyati

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引用次数: 0

Abstract

Thalassiosira sp. adalah diatom yang digunakan sebagai pakan alami bagi larva udang vanamei. Thalassiosira sp. mempunyai kandungan protein sekitar 44,5 %,karbohidrat 26,1% dan lemak 11,8% dari berat keringnya. Thalassiosira sp. adalah salah satu pakan alami yang baik karena mempunyai nilai nutrisi yang tinngi serta kandungan silika yang bagus untuk larva udang vanamei. Pertumbuhan diatom dibutuhkan media nutrisi dalam mendukung pertumbuhan plankton harus mengandung silika untuk mendukung pertumbuhan dan memperkuat cangkang sel. Penelitian ini bertujuan untuk mengetahui konsentrasi silikat yang tepat guna mengoptimalkan pertumbuhan kepadatan dan produksi pigmen klorofil pada Thalasiosira sp. Metode yang digunakan adalah eksperimen laboratorium dengan menggunakan Rancangan Acak Lengkap. Mikroalga Thalassiosira sp di kultivasi dengan lima taraf perlakuan konsentrasi silikat yang berbeda yaitu 0 ppm, 12 ppm,15 ppm, 18 ppm dan 21 ppm dengan tiga ulangan. Pertumbuhan Thalasiosira sp. diamati selama 13 x 24 jam kemudian dipanen untuk perhitungan biomassanya. Biomassa basah hasil kultivasi diekstraksi menggunakan pelarut aseton PA. Ekstrak aseton Thalassiosira sp. kemudian dianalisis kandungan pigmen klorofilnya dengan spektrofotometri. Hasil densitas tertinggi terdapat pada konsentrasi (15 ppm) Kandungan Klorofil Thalassiosira sp. tertinggi diproduksi pada konsentrasi (15 ppm) yaitu 184 µg/mL. Berdasarkan hasil penelitian dapat disimpulkan bahwa konsentrasi silikat berpengaruh secara signifikan terhadap kandungan klorofil Thalassiosira sp. Thalassiosira sp is a diatom that is used as natural food for vanamei shrimp larvae. Thalassiosira sp. It has a protein content of about 44.5%, carbohydrates 26.1% and fat 11.8% of its dry weight. Thalassiosira sp. is one of the good natural feeds because it has high nutritional value and good silica content for vanamei shrimp larvae. The growth of diatoms requires a nutrient medium to support the growth of plankton. It must contain silica to support growth and strengthen cell shells. This study aims to determine the appropriate concentration of silicate in order to optimize the growth of density and production of chlorophyll pigment in Thalasiosira sp. The method used is a laboratory experiment using a completely randomized design. The microalgae Thalasiosira sp was cultivated with five different levels of silicate concentration treatment, namely 0 ppm, 12 ppm, 15 ppm, 18 ppm and 21 ppm with three replications. Growth of Thalasiosira sp. observed for 13 x 24 hours and then harvested for biomass calculation. The wet biomass from the cultivation was extracted using acetone PA as a solvent. Thalasiosira sp. acetone extract. then analyzed the content of chlorophyll pigment by spectrophotometry. The highest density results were found at the concentration (15 ppm) of the Chlorophyll content of Thalassiosira sp. The highest was produced at a concentration (15 ppm) which was 184 g/mL. Based on the results of the study, it can be concluded that the salinity treatment had a significant effect on the chlorophyll content of Thalasiosira sp.

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不同硅酸盐浓度下的叶绿素-a 含量和硅藻密度分析

Thalassiosira sp.是一种硅藻，它是万年青幼虫的主要食物来源。它的蛋白质含量为 44.5%，碳水化合物含量为 26.1%，钙含量为 11.8%。硅藻是一种非常适合万年青幼虫生长的大型硅藻，它不仅能提供丰富的营养，还能提供丰富的蛋白质。硅藻是一种营养介质，它能促进浮游生物的生长，还能促进硅胶的生长，并提高硅胶的吸附力。该研究的目的是建立一个硅胶中心，以优化 Thalasiosira sp.的浮游生物和光油色素的生产。研究采用的方法是在实验室中进行试验，以确定是否能生产 Rancangan Acak Lengkap。在硅胶中心的土壤中生长的沙雷氏菌的浓度分别为百万分之 0、百万分之 12、百万分之 15、百万分之 18 和百万分之 21，最高浓度分别为百万分之 0、百万分之 12、百万分之 15、百万分之 18 和百万分之 21。Thalasiosira sp.的直径为 13 x 24 焦耳，因此其生物量也会增加。这些生物质可用于生产褐藻粉。通过荧光光度计，可以观察到Thalassiosira sp.的色素沉积。在浓度为 15 ppm 时，其密度为 184 µg/mL。这表明，硅胶浓度对 Thalassiosira sp. Thalassiosira sp 是一种硅藻，是凡纳美对虾幼体的天然食物。其蛋白质含量约为干重的 44.5%，碳水化合物含量为 26.1%，脂肪含量为 11.8%。硅藻具有很高的营养价值和良好的二氧化硅含量，对凡纳滨对虾幼体来说是一种很好的天然饲料。硅藻的生长需要营养介质来支持浮游生物的生长。它必须含有二氧化硅，以支持生长和强化细胞外壳。本研究旨在确定适当的硅酸盐浓度，以优化 Thalasiosira sp.的生长密度和叶绿素色素的产生。微藻类 Thalasiosira sp 的培养采用了五种不同的硅酸盐浓度处理水平，即 0 ppm、12 ppm、15 ppm、18 ppm 和 21 ppm，三次重复。观察 Thalasiosira sp.生长 13 x 24 小时，然后收获计算生物量。以丙酮 PA 为溶剂提取培养的湿生物量。然后用分光光度法分析叶绿素色素的含量。在叶绿素含量为 184 克/毫升的浓度（15 ppm）下，Thalassiosira sp.的密度结果最高。根据研究结果可以得出结论，盐度处理对 Thalasiosira sp.的叶绿素含量有显著影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Marine Research 地学-海洋学

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： The Journal of Marine Research publishes peer-reviewed research articles covering a broad array of topics in physical, biological and chemical oceanography. Articles that deal with processes, as well as those that report significant observations, are welcome. In the area of biology, studies involving coupling between ecological and physical processes are preferred over those that report systematics. Authors benefit from thorough reviews of their manuscripts, where an attempt is made to maximize clarity. The time between submission and publication is kept to a minimum; there is no page charge.