Molecular Detection and Identification of oprl and lasb Genes Isolated from Pseudomonas aeruginosa

Abstract

: Species Pseudomonas aeruginosa is a distinguished opportunistic nosocomial bacterium that is commonly dispersed in nature. A pathogen that causes a large range of infections i.e. chronic as well as acute, with considerable levels of mortality and morbidity. Infections of P. aeruginosa are extremely hard to eliminate because of the presence of several virulence determinants as well as the intrinsic resistance against antibiotics. The present study investigated the detection of the virulence genes by polymerase chain reaction (PCR) namely oprL and lasB in the P. aeruginosa that were isolated detection from both human and animal specimens. 120 specimens of P. aeruginosa including samples were then collected from hospitals and veterinary laboratories, from which 55 isolates of P. aeruginosa were identified and selected. Pseudomonas aeruginosa strains were present in 40% of animal sample isolates and 86% of human sample isolates. The PCR results showed that oprL genes were detected in 54.16% of isolates obtained from animal samples and 80.76% of isolates obtained from human samples. While, the lasB gene was present at 58.33% and 92.30% in the isolates obtained from animal and human samples, respectively. Our results also showed the coexistence of both virulence genes in 32.34% of animal sample isolates and 25.10% of human sample isolations. The detection of gene oprL and lasB by the PCR technique can be effectively used for the molecular-level identification of P. aeruginosa. The identification of such virulence genes in P. aeruginosa isolates suggests that these may be linked with varying degrees of the pathogenesis and inherent virulence in this pathogen.