Isolasi dan Uji Aktivitas Antioksidan Minyak Atsiri Daging Buah Pala (Myristica fragrans)

Muh Nasir, Eri Marwati
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Abstract

Nutmeg is considered an important ingredient of various industrial applications ranging from food to cosmetics. The pharmaceutical product is also important for its antioxidant and antimicrobial properties. Further research is needed to isolate and identify the bioactive compounds of Myristica fragrans. The aim of this research was to isolate and determine the antioxidant activity of the essential oil of nutmeg flesh (Myristica fragrans). The sample used was nutmeg flesh (Myristica fragrans) taken from Ternate Island, North Maluku. The essential oil of nutmeg flesh was isolated using water vapor distillation and the antioxidant activity was tested using the DPPH method. All data are presented as mean ± standard deviation (SD) and were carried out in triplicate. The results of the research showed that the isolation of nutmeg essential oil obtained with a yield of 0,32%. Organoleptically, the essential oil of nutmeg flesh obtained from the steam distillation process has quality that meets SNI, in the form of a clear to rusty liquid and has a distinctive odor. Phytochemical tests obtained that the essential oil of nutmeg flesh contains alkaloids, flavonoids and steroids, and does not contain polyphenols and saponins. The concentration of essential oil increases, the absorption value and percent inhibition increase so that an average IC50 value of 49,34 ppm is obtained. The essential oil of nutmeg flesh by steam distillation obtained a yield of 0,32% and has antioxidant activity which is classified as a strong antioxidant
肉豆蔻(Myristica fragrans)果肉精油的分离和抗氧化活性测试
肉豆蔻被视为从食品到化妆品等各种工业应用的重要成分。这种药品还具有抗氧化和抗菌特性。需要进一步研究分离和鉴定肉豆蔻的生物活性化合物。本研究的目的是分离和确定肉豆蔻肉(Myristica fragrans)精油的抗氧化活性。使用的样本是取自北马鲁古特尔纳特岛的肉豆蔻肉(Myristica fragrans)。使用水蒸气蒸馏法分离出肉豆蔻肉的精油,并使用 DPPH 法测试其抗氧化活性。所有数据均以平均值±标准偏差(SD)表示,一式三份。研究结果表明,肉豆蔻精油的分离率为 0.32%。从有机化学角度看,通过蒸汽蒸馏工艺获得的肉豆蔻肉精油质量符合 SNI 标准,呈透明至铁锈色液体状,具有独特的气味。植物化学测试表明,肉豆蔻果肉精油含有生物碱、黄酮类和类固醇,不含多酚和皂苷。随着精油浓度的增加,吸收值和抑制百分比也随之增加,因此平均 IC50 值为 49.34 ppm。通过蒸汽蒸馏法提取的肉豆蔻肉精油产量为 0.32%,具有抗氧化活性,被归类为强抗氧化剂。
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