In Vitro Wound-healing Activity of Ziziphus jujuba and Its Anti-inflammatory Effects in Rats

Proceedings of the Bulgarian Academy of Sciences Pub Date : 2024-01-29 DOI:10.7546/crabs.2024.01.14

Insaf Mansour, C. Ozay, Fadime Kose, Soraya Rahmanı, Omar Kharoubı

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Abstract

Jujube (Ziziphus jujuba) has a long history of use in traditional medicine. It has many pharmacological properties, including neuroprotective, antioxidative, hepatoprotective, anti-cancer, anti-inflammatory, and immunomodulatory. Thus, this study aimed to evaluate the anti-inflammatory activity of the ethanolic extract of Ziziphus (EEZ) fruit and its wound-healing effect. The anti-inflammatory activity of EEZ was assessed in vivo by carrageenan-induced paw edema test. NIH-3T3 mouse embryonic fibroblast cells were used to evaluate the wound healing effect of EEZ. The effect of the extract on wound-healing was examined by in vitro scratch test. Also, the level of C-reactive protein (CRP) in the serum of rats was determined using the CRP latex test kit. The ethanol extract of jujube fruit induced wound closure in NIH-3T3 fibroblast cells for 18 h (88.5%) at 1 μ\umu g/mL concentration. Also, EEZ significantly inhibited carrageenan-induced edema in rat paws. However, a statistically significant decrease in CRP levels was observed in rats pre-treated with EEZ. As a result, Z. jujuba fruit extract had anti-inflammatory and wound healing effects, which might be strongly related to its phytochemical composition.

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酸枣仁的体外伤口愈合活性及其对大鼠的抗炎作用

大枣（酸枣仁）在传统医学中的应用历史悠久。它具有多种药理特性，包括神经保护、抗氧化、保肝、抗癌、抗炎和免疫调节。因此，本研究旨在评估酸枣仁乙醇提取物的抗炎活性及其伤口愈合效果。EEZ 的抗炎活性通过卡拉胶诱导的爪水肿试验进行体内评估。用 NIH-3T3 小鼠胚胎成纤维细胞评估 EEZ 的伤口愈合效果。通过体外划痕试验检测了提取物对伤口愈合的影响。此外，还使用 CRP 胶乳检测试剂盒测定了大鼠血清中 C 反应蛋白（CRP）的水平。在1 μumu g/mL浓度下，枣果乙醇提取物可诱导NIH-3T3成纤维细胞伤口闭合18小时（88.5%）。此外，EEZ 还能明显抑制卡拉胶诱导的大鼠爪部水肿。然而，用 EEZ 预处理的大鼠的 CRP 水平出现了统计学意义上的明显下降。因此，酸枣仁提取物具有抗炎和伤口愈合的作用，这可能与其植物化学成分密切相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Proceedings of the Bulgarian Academy of Sciences

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