Hepatoprotective Effect of Extra Virgin Olive Oil and Apple Cider Vinegar in Type-2 Diabetic Rat Model

Proceedings Pub Date : 2024-01-31 DOI:10.47489/szmc.v38i1.423

Ruqaiyya Nazir, Muniza Saeed, Ghulam Mustafa, Seher Naeem, Hifza Noor Lodhi, Sehrish Ijaz

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Abstract

Introduction: Type-2 diabetes mellitus, a major public health problem, is caused by insulin resistance. In diabetics, chronic hyperglycemia results in formation of reactive oxygen species. Oxidative stress in diabetic patients is responsible for hepatic damage because it causes activation of several pro-inflammatory mediators in liver. Hepatic complications of diabetes include fatty liver disease, necrosis, cirrhosis, carcinoma and liver failure. Aims & Objectives: To determine and compare the hepatoprotective effect of extra virgin olive oil and apple cider vinegar in streptozotocin induced Type-2 diabetic rat model. Place and Duration of Study: This experimental study was conducted at Animal House of Postgraduate Medical Institute, Lahore, Pakistan from May 2021 to June 2021. Material & Methods: In this study, 40 male Sprague Dawley rats were divided into 4 groups i.e., Group I was NC (negative control), Group II PC (positive control), Group III EVOO (Extra virgin olive oil) and Group IV (Apple cider vinegar), each group having 10 rats. Diabetes was induced in all rats except the rats of NC group at the start of the study by intraperitoneal administration of injection nicotinamide, followed by injection STZ after 15 minutes. Group III was given 1ml/100gBW/ day EVOO and Group IV was given 2ml/kgBW/day diluted ACV with distilled water in 1:5 orally for 4 weeks. Terminal sampling was performed at the end of the 4th week for estimation of liver enzymes (ALT, ALP and AST) in serum. Data were entered and analyzed in SPSS version 26. p value of ? 0.05 was considered significant. Results: On comparison with PC group, both the treatment groups showed significant decrease in serum ALT levels with p values 0.026 for EVOO and < 0.001 for ACV. Serum ALP levels were also decreased significantly in both the treated groups with p value < 0.001 for EVOO as well as for the ACV group. However, reduction in serum AST levels was nonsignificant with p values 0.082 and 0.058 in EVOO and ACV groups respectively. Conclusion: Both EVOO and ACV have hepatoprotective effect in Type-2 diabetic rats. However, ACV is more effective.

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特级初榨橄榄油和苹果醋对 2 型糖尿病大鼠肝脏的保护作用

简介2 型糖尿病是一个主要的公共健康问题，是由胰岛素抵抗引起的。糖尿病患者长期高血糖会形成活性氧。糖尿病患者体内的氧化应激会导致肝损伤，因为它会激活肝脏中的多种促炎介质。糖尿病的肝脏并发症包括脂肪肝、肝坏死、肝硬化、肝癌和肝衰竭：确定并比较特级初榨橄榄油和苹果醋在链脲佐菌素诱导的 2 型糖尿病大鼠模型中的保肝作用：本实验研究于 2021 年 5 月至 2021 年 6 月在巴基斯坦拉合尔研究生医学院动物房进行：本研究将 40 只雄性 Sprague Dawley 大鼠分为 4 组，即 I 组 NC（阴性对照）、II 组 PC（阳性对照）、III 组 EVOO（特级初榨橄榄油）和 IV 组（苹果醋），每组 10 只。除 NC 组大鼠外，其他大鼠均在研究开始时腹腔注射烟酰胺诱发糖尿病，15 分钟后注射 STZ。第 III 组大鼠每天每 100 克体重口服 1 毫升乙二酸；第 IV 组大鼠每天每公斤体重口服 2 毫升乙酸乙酯与蒸馏水按 1:5 稀释的乙酸乙酯，连续口服 4 周。第 4 周结束时进行最终采样，以估算血清中的肝酶（ALT、ALP 和 AST）。数据用 SPSS 26 版进行输入和分析，P 值在 0.05 以内为显著：结果：与 PC 组相比，两个治疗组的血清 ALT 水平都有明显下降，EVOO 的 p 值为 0.026，ACV 的 p 值小于 0.001。两个治疗组的血清 ALP 水平也明显下降，EVOO 和 ACV 组的 P 值均小于 0.001。然而，EVOO 组和 ACV 组的血清 AST 水平下降不明显，P 值分别为 0.082 和 0.058：结论：EVOO 和 ACV 对 2 型糖尿病大鼠均有保肝作用。结论：EVOO 和 ACV 对 2 型糖尿病大鼠都有保肝作用，但 ACV 的效果更好。

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