MORPHOFUNCTIONAL CHANGES IN THE ORGANS OF THE IMMUNE SYSTEM OF RATS DURING VARIOUS METHODS OF LOCAL TREATMENT OF PURULENT WOUNDS IN THE BACKGROUND OF ALLOXAN DIABETES

Abstract

Gangrene of the limb and purulent-inflammatory processes against the background of diabetes mellitus have the largest share among surgical diseases. The purpose of the study is to identify morphofunctional changes in the organs of the immune system under experimental conditions when using various methods of treating purulent wounds when modeling alloxan diabetes. Experimental studies were carried out on 150 animals (white outbred rats), in which purulent wounds were simulated against the background of alloxan diabetes. In control I group, the nature of the course of the purulent-inflammatory process was studied without correction of treatment, in control II group, purulent wounds were treated with miliacil, in control III group, EHF therapy was used. For local treatment of wounds of the main group of experimental animals, EHF therapy and miliacyl were used. In addition to taking into account clinical data, mesenteric lymph nodes, thymus, and pancreas were examined in animals of all groups after euthanasia. The most favorable treatment results were observed in experimental animals of the main group. As a result of a histological study of the thymus, atrophy of the thymic parenchyma (cortex and medulla) was discovered, combined with hyperplasia of the interlobar perivascular spaces with a simultaneous increase in the proportion of adipose tissue. The phenomenon of accidental involution of the thymus was less pronounced in animals of the main group than in rats of control groups. Morphofunctional changes in the organs of the immune system were established with various methods of treating purulent wounds against the background of alloxan diabetes. The developed new treatment method inhibits (limites) the involution of the thymus gland, corrects the humoral component of immunity and ensures optimization of reparative processes in the wound area.