Aldolase: A Desirable Biocatalytic Candidate for Biotechnological Applications

IF 3.8 3区化学 Q2 CHEMISTRY, PHYSICAL

Catalysts Pub Date : 2024-01-31 DOI:10.3390/catal14020114

Moloko G. Mathipa-Mdakane, Lucia Steenkamp

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引用次数: 0

Abstract

The utilization of chemical reactions is crucial in various industrial processes, including pharmaceutical synthesis and the production of fine chemicals. However, traditional chemical catalysts often lack selectivity, require harsh reaction conditions, and lead to the generation of hazardous waste. In response, biocatalysis has emerged as a promising approach within green chemistry, employing enzymes as catalysts. Among these enzymes, aldolases have gained attention for their efficiency and selectivity in catalyzing C-C bond formation, making them versatile biocatalysts for diverse biotechnological applications. Despite their potential, challenges exist in aldolase-based biocatalysis, such as limited availability of natural aldolases with desired catalytic properties. This review explores strategies to address these challenges, including immobilization techniques, recombinant expression, and protein engineering approaches. By providing valuable insights into the suitability of aldolases as biocatalysts, this review lays the groundwork for future research and the exploration of innovative strategies to fully harness the potential of aldolases in biotechnology. This comprehensive review aims to attract readers by providing a comprehensive overview of aldolase-based biocatalysis, addressing challenges, and proposing avenues for future research and development.

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醛缩酶：生物技术应用中理想的生物催化候选物

化学反应的利用在各种工业流程（包括医药合成和精细化学品生产）中至关重要。然而，传统的化学催化剂往往缺乏选择性，需要苛刻的反应条件，并且会产生有害废物。为此，生物催化成为绿色化学中一种前景广阔的方法，它采用酶作为催化剂。在这些酶中，醛醇酶因其催化 C-C 键形成的效率和选择性而备受关注，成为可用于多种生物技术应用的多功能生物催化剂。尽管醛醇酶具有潜力，但基于醛醇酶的生物催化仍面临挑战，例如具有理想催化特性的天然醛醇酶的可用性有限。本综述探讨了应对这些挑战的策略，包括固定化技术、重组表达和蛋白质工程方法。通过对醛醇酶作为生物催化剂的适用性提供有价值的见解，本综述为未来的研究和探索创新战略奠定了基础，以充分利用醛醇酶在生物技术中的潜力。这篇综合性综述旨在通过全面概述基于醛缩酶的生物催化、应对挑战以及提出未来研究和开发的途径来吸引读者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Catalysts CHEMISTRY, PHYSICAL-

CiteScore

6.80

自引率

7.70%

发文量

1330

审稿时长

3 months

期刊介绍： Catalysts (ISSN 2073-4344) is an international open access journal of catalysts and catalyzed reactions. Catalysts publishes reviews, regular research papers (articles) and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.