Hydrangea serrata extract exerts tumor inhibitory activity against hepatocellular carcinoma HepG2 cells via inducing p27/CDK2-mediated cell cycle arrest and apoptosis

IF 1.7 4区 医学 Q3 TROPICAL MEDICINE
Ye-eun Kim, Jeonghye Hwang, Ki-Young Kim
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引用次数: 0

Abstract

To examine the inhibitory effect of Hydrangea serrata extract against hepatocellular carcinoma HepG2 cells and its underlying mechanisms. The effects of Hydrangea serrata extract on growth inhibition of tumor cells and spheroids were assessed using MTT and 3D culture assays. Quantitative real-time PCR and Western blot analyses were employed to investigate the changes in mRNA and protein expression levels of molecules related to cell cycle and apoptosis. Hydrangea serrata extract effectively inhibited the growth of both tumor cells and spheroids. The extract also significantly upregulated p27 mRNA expression and downregulated CDK2 mRNA expression, leading to cell cycle arrest. Moreover, increased BAX/Bcl-2 ratio as well as caspase-9 and - 3 were observed after treatment with Hydrangea serrata extract, indicating the induction of tumor cell apoptosis. Hydrangea serrata extract has the potential to alleviate tumors by effectively modulating cell-cycle-related gene expressions and inducing apoptosis, thereby inhibiting tumor growth.
绣球花提取物通过诱导 p27/CDK2 介导的细胞周期停滞和细胞凋亡,对肝癌 HepG2 细胞具有肿瘤抑制活性
研究绣球花提取物对肝癌 HepG2 细胞的抑制作用及其内在机制。 采用 MTT 和三维培养法评估绣球花提取物对肿瘤细胞和球形细胞生长的抑制作用。实时定量 PCR 和 Western 印迹分析用于研究细胞周期和细胞凋亡相关分子的 mRNA 和蛋白质表达水平的变化。 绣球花提取物能有效抑制肿瘤细胞和球形细胞的生长。绣球花提取物还能明显上调 p27 mRNA 的表达,下调 CDK2 mRNA 的表达,从而导致细胞周期停滞。此外,经绣球花提取物处理后,还观察到 BAX/Bcl-2 比率以及 caspase-9 和 - 3 增加,这表明绣球花提取物能诱导肿瘤细胞凋亡。 绣球花提取物可有效调节细胞周期相关基因的表达,诱导细胞凋亡,从而抑制肿瘤生长,具有缓解肿瘤的潜力。
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来源期刊
Asian Pacific journal of tropical biomedicine
Asian Pacific journal of tropical biomedicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
3.10
自引率
11.80%
发文量
2056
审稿时长
4 weeks
期刊介绍: The journal will cover technical and clinical studies related to health, ethical and social issues in field of biology, bacteriology, biochemistry, biotechnology, cell biology, environmental biology, microbiology, medical microbiology, pharmacology, physiology, pathology, immunology, virology, toxicology, epidemiology, vaccinology, hematology, histopathology, cytology, genetics and tropical agriculture. Articles with clinical interest and implications will be given preference.
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