Response of carbon sources and their various concentrations on in vitro rooting of banana cv. Chinichampa (AAB) through shoot tip ​

Abstract

In vitro propagated banana plants are steadily becoming the planting material of choice because of disease free, uniformity and the possibility of rapid multiplication. The present investigation was carried out at the tissue culture laboratory, Department of Horticulture, School of Agricultural Sciences, Nagaland University, Medziphema campus during the year 2019-2022. Data recorded for different parameters viz. days to greening, days required for multiple buds’ initiation, number of multiple buds produced per explant and length of multiple buds after 30 days were subjected to completely randomized design (CRD). The present study revealed the importance of various carbon sources and their role in micropropagation of banana through shoot tip culture. The minimum days required for root initiation were observed in root induction media (RIM) supplemented with 30g glucose. Rooting percentage (%), number of roots per shoot and length of longest roots (cm) after 30 days was seen in RIM supplemented with table sugar at 40g, 30g and 20g respectively. Meanwhile maximum average length of shoot (cm) after 30 days was observed in RIM supplemented with 40g glucose and highest number of leaves per plantlets was recorded in RIM supplemented with 30g sucrose. The results indicate the significance of carbon sources in micropropagation of banana. They improved plant regeneration and facilitated rapid multiplication of disease and virus free banana plantlets.