RHIZOBACTERIA AS ANTAGONIST AGAINST Fusarium oxysporum CAUSING TOMATO WILT

Noor Muhammad Shah, Syed Zulfiqar Ali, Muhammad Waris, Atta Ullah, Jabeen Jabeen, B. Ahmed, Sana Shazia Jiskani
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Abstract

Tomatoes (Lycopersicon esculentum L.) are the most extensively grown vegetable in the world. It belongs to the Solanaceae family and is commonly planted for its tasty fruits. Pests, weeds, diseases, and parasites are just a few of the numerous variables that significantly affect tomato growth and yield. The most common disease affecting tomatoes is fusarium wilt. Fifteen rhizobacterial strains were identified by morphological and biochemical analyses in this work, and they were employed as an antagonist against Fusarium oxysporum f.sp. lycopersici. Fifteen isolates were investigated for their antagonistic properties against Fusarium oxysporum f.sp. lycopersici using an invitro dual culture approach. The growth of Fusarium oxysporum f.sp. lycopersici was suppressed by each isolate. Out of the 15 rhizobacteria isolates, isolate RBS-5 exhibited the highest level of growth inhibition and strongly suppressed the development of Fusarium oxysporum f.sp. lycopersici, resulting in a 57.28 percent reduction in pathogen growth as compared to the control. The development of Fusarium oxysporum f.sp. lycopersici was suppressed by isolates RBS-12, RBS-6, and RBS-15, in decreasing order of merit, compared to the control by 53.7, 51.91, and 51.73 percent. Isolate RBS-13 showed the least amount of pathogen growth inhibition 20.83 percent. The data was statistically analyzed.
RHIZOBACTERIA 作为抗菌剂,对抗引起番茄枯萎病的 Fusarium oxysporum 菌
番茄(Lycopersicon esculentum L.)是世界上种植最广泛的蔬菜。它属于茄科植物,通常因其美味的果实而被种植。害虫、杂草、疾病和寄生虫只是对番茄生长和产量有重大影响的众多变量中的一小部分。影响番茄的最常见疾病是镰刀菌枯萎病。在这项工作中,通过形态学和生化分析鉴定了 15 株根瘤菌,并将它们用作抗镰刀菌 Fusarium oxysporum f.sp. lycopersici 的拮抗剂。采用无菌双重培养法研究了 15 个分离菌株对镰孢属茄科菌的拮抗特性。每种分离物都抑制了 Fusarium oxysporum f.sp. lycopersici 的生长。在 15 个根瘤菌分离物中,分离物 RBS-5 的生长抑制水平最高,能强烈抑制 Fusarium oxysporum f.sp. lycopersici 的生长,与对照相比,病原体的生长减少了 57.28%。分离物 RBS-12、RBS-6 和 RBS-15 依次抑制了 Fusarium oxysporum f.sp. lycopersici 的生长,抑制率分别为 53.7%、51.91% 和 51.73%。菌株 RBS-13 对病原体生长的抑制作用最小,仅为 20.83%。对数据进行了统计分析。
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