The Effect of HIF1-α/NF-κB/MMP2 Signaling Pathways Mediated by ALDH2 on Chronic Intermittent Hypoxia Induced Myocardial Injury in Rats

International Journal of Biology and Life Sciences Pub Date : 2024-02-22 DOI:10.54097/wxcz8y41

Bin Chen

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Abstract

(1) Objective: To investigate the effect of HIF1-α/NF-κB/MMP2 signaling pathways mediated by ALDH2 on chronic intermittent hypoxia induced myocardial injury in rats. (2) Methods: Thirty SD rats were randomly assigned to three groups (10 rats in each group): control group (CONTROL), chronic intermittent hypoxia group (CIH), and CIH + ALDH2 group. Rats in the CIH + ALDH2 group were established by an intraperitoneal injection of the ALDH2 activator, Alda-1 (20 mg/kg), once a day for three consecutive days. After three months, changes in ventricular function were determined by ultrasound. The expressions of HIF1-α, NF-κB, and MMP2 proteins were detected by protein blotting, and the concentration of 4-HNE in myocardial tissue was measured. (3) Results: Compared with the control group, echocardiography showed that rats in the CIH group had significantly reduced myocardial function, elevated protein levels of HIF1-α, NF-κB, and MMP2 in myocardial tissues, and increased the expression of 4-HNE in myocardial tissues (P<0.01); Compared with the CIH group, rats in the CIH + ALDH2 group showed significant improvement in myocardial function, decreased protein levels of HIF1-α, NF-κB, and MMP2 in myocardial tissue, and decreased the expression of 4-HNE in myocardial tissue (P<0.01). (4) Conclusion: Chronic intermittent hypoxia increases the expression of HIF1-α to damage the myocardium and affect cardiac function, and ALDH2 inhibits the expression of HIF1-α to protect the myocardium.

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ALDH2 介导的 HIF1-α/NF-κB/MMP2 信号通路对慢性间歇性缺氧诱导的大鼠心肌损伤的影响

(1) 目的研究ALDH2介导的HIF1-α/NF-κB/MMP2信号通路对慢性间歇性缺氧诱导的大鼠心肌损伤的影响。(2）方法：将 30 只 SD 大鼠随机分为三组（每组 10 只）：对照组（CONTROL）、慢性间歇性缺氧组（CIH）和 CIH + ALDH2 组。CIH + ALDH2 组大鼠腹腔注射 ALDH2 激活剂 Alda-1（20 毫克/千克），每天一次，连续注射三天。三个月后，通过超声波测定心室功能的变化。通过蛋白印迹检测 HIF1-α、NF-κB 和 MMP2 蛋白的表达，并测定心肌组织中 4-HNE 的浓度。(3）结果：与对照组相比，超声心动图显示 CIH 组大鼠心肌功能明显降低，心肌组织中 HIF1-α、NF-κB 和 MMP2 蛋白水平升高，心肌组织中 4-HNE 表达增加（P<0.01）；与CIH组相比，CIH + ALDH2组大鼠心肌功能明显改善，心肌组织中HIF1-α、NF-κB、MMP2蛋白水平降低，心肌组织中4-HNE表达降低（P<0.01）。(4）结论：慢性间歇性缺氧会增加 HIF1-α 的表达，损伤心肌，影响心功能，而 ALDH2 可抑制 HIF1-α 的表达，保护心肌。

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International Journal of Biology and Life Sciences

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