DETECTION OF GENES RESPONSIBLE FOR HEAVY METALS RESISTANCE IN LOCALLY ISOLATED PSEUDOMONAS SPP.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-02-25 DOI:10.36103/wgz9vb91

Al-Sajad M. S H.A.A. Alsalim, Researcher Prof.

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引用次数: 0

Abstract

Plant growth-promoting rhizobacteria (PGPR) that can tolerate heavy metals, provide the basis for microbial inoculums showing heavy metals tolerance properties. This study was aimed to detect the heavy metal resistance genes in plant-growth-promoting Pseudomonas spp. isolated from many agricultural fields. The collected isolates were screened for their plant growth-promoting (PGP) traits, hydrolytic enzymes, Siderophore, ammonia, and indole-3-acetic acid (IAA). Then, subjected to concentrations of CuSO4, CdCl2, and ZnCl2 to determine the minimum inhibitory concentration (MIC). The DNA was extracted from the selected isolates then PCR test was achieved to detect copA, copB, and czcA genes, responsible for heavy metal resistance. Seventy Pseudomonas spp. isolates were obtained; 41 (58.57%), 6 (8.57%), and 15 (21.42%) isolate produced protease, cellulase, and pectinase, respectively. The isolates were positive for siderophore and ammonia production. However, 68 (97.14%) isolates have produced indole-3-acetic acid. Eight isolates were selected and identified as Pseudomonas aeruginosa using the Vitek 2 compact system. The isolates' resistance to heavy metals differed significantly. The isolate B49 had a higher resistance to CuSO4 (MIC = 3200 µg/ml) and ZnCl2 (MIC = 2600 µg/ml), while the isolate B66 recorded a higher resistance to CdCl2 (MIC = 1000 µg/ml). copB, and czcA genes were detected in the eight P. aeruginosa isolates, while copA gene was detected in seven, except B69.

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在本地分离的假单胞菌中检测重金属抗性基因

能耐受重金属的植物生长促进根瘤菌（PGPR）为微生物接种物提供了耐重金属特性的基础。本研究旨在检测从许多农田中分离出来的促进植物生长假单胞菌中的重金属抗性基因。对收集到的分离菌株进行了植物生长促进（PGP）性状、水解酶、嗜苷酸、氨和吲哚-3-乙酸（IAA）的筛选。然后，用硫酸铜（CuSO4）、氯化镉（CdCl2）和氯化锌（ZnCl2）浓度测定最低抑菌浓度（MIC）。从所选分离菌株中提取 DNA，然后进行 PCR 检测，以检测产生重金属抗性的 copA、copB 和 czcA 基因。共分离出 70 株假单胞菌，其中 41 株（58.57%）、6 株（8.57%）和 15 株（21.42%）分别产生蛋白酶、纤维素酶和果胶酶。这些分离物对嗜苷酸盐和氨的产生呈阳性。但有 68 个（97.14%）分离菌株产生吲哚-3-乙酸。使用 Vitek 2 紧凑型系统筛选出 8 个分离株，并鉴定其为铜绿假单胞菌。分离物对重金属的抗性差异显著。分离物 B49 对 CuSO4（MIC = 3200 µg/ml）和 ZnCl2（MIC = 2600 µg/ml）的抗性较高，而分离物 B66 对 CdCl2（MIC = 1000 µg/ml）的抗性较高。在 8 个铜绿假单胞菌分离物中检测到了 copB 和 czcA 基因，而在 7 个分离物（B69 除外）中检测到了 copA 基因。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.