The Anti-proliferative Effect of Caffeic Acid and Dactolisib on Human Cervical Carcinoma HeLa Cell Line

Cumhuriyet Science Journal Pub Date : 2024-02-27 DOI:10.17776/csj.1394831

Zeynep Busra Bolat

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Abstract

Cervical carcinoma is a common gynecological cancer with high mortality rate among women worldwide. Caffeic acid exerts an antiproliferative effect against cervical carcinoma. Dactolisib is a dual PI3K and mTOR inhibitor that has a therapeutic potential for cervical carcinoma. This study aimed to reveal the anti-proliferative effect of combination treatment of caffeic acid and Dactolisib on cervical carcinoma HeLa cell line. Cytotoxicity of caffeic acid and Dactolisib on HeLa cell line was assessed by MTS assay. Colony formation of HeLa cells treated with caffeic acid and Dactolisib was determined by staining colonies with crystal violet and visualizing under light microscope. Dactolisib decreased cell proliferation of HeLa cells in time and dose dependent manner. 5 μM caffeic acid did not show any significant change in cell viability of HeLa cells. Combination treatment of 5 μM caffeic acid and 0.5 μM Dactolisib showed decrease in cell viability of HeLa cells when compared to Dactolisib treated cells. Combination of caffeic acid and Dactolisib decreased colony diameter of HeLa cells significantly when compared to control group. Caffeic acid and Dactolisib shows anti-proliferative effect on human cervical carcinoma HeLa cell line, so further studies should be performed to reveal the mechanism of action.

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咖啡酸和 Dactolisib 对人宫颈癌 HeLa 细胞株的抗增殖作用

宫颈癌是一种常见的妇科癌症，在全世界妇女中死亡率很高。咖啡酸对宫颈癌具有抗增殖作用。Dactolisib是一种PI3K和mTOR双重抑制剂，具有治疗宫颈癌的潜力。本研究旨在揭示咖啡酸和 Dactolisib 联合治疗对宫颈癌 HeLa 细胞株的抗增殖作用。研究采用 MTS 法评估了咖啡酸和 Dactolisib 对 HeLa 细胞株的细胞毒性。用结晶紫对HeLa细胞进行染色，并在光镜下观察其菌落形成情况。Dactolisib可减少HeLa细胞的增殖，且具有时间和剂量依赖性。5 μM 咖啡酸对 HeLa 细胞的活力没有明显影响。与 Dactolisib 处理的细胞相比，5 μM 咖啡酸和 0.5 μM Dactolisib 的联合处理会降低 HeLa 细胞的活力。与对照组相比，咖啡酸和 Dactolisib 的组合能显著减少 HeLa 细胞的集落直径。咖啡酸和 Dactolisib 对人宫颈癌 HeLa 细胞株具有抗增殖作用，因此应进一步研究其作用机制。

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来源期刊

Cumhuriyet Science Journal

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审稿时长

10 weeks