An in Vitro Approach: Antibacterial Activity of Sansevieria trifasciata Prain. Leaves with Chemometric Analysis

Jurnal Sains dan Kesehatan Pub Date : 2024-02-29 DOI:10.25026/jsk.v6i1.2087

Henny Kasmawati, N. A. Sida, A. Arfan, Wa Ode Dian Indrayanti, Arman Rusman

{"title":"An in Vitro Approach: Antibacterial Activity of Sansevieria trifasciata Prain. Leaves with Chemometric Analysis","authors":"Henny Kasmawati, N. A. Sida, A. Arfan, Wa Ode Dian Indrayanti, Arman Rusman","doi":"10.25026/jsk.v6i1.2087","DOIUrl":null,"url":null,"abstract":"Exploration the antibacterial activity of S.trifasciata Prain. is still limited, therefore this study aims to assess the antibacterial activity of extracts and ethyl acetate fractions of Sansevieria trifasciata Prain. The S.trifasciata leaves was macerated with ethanol 96%, then fractionated using the trituration method with ethyl acetate. The treatment group was divided into positive control group (PC) using ciprofloxacin, negative control (NC) using DMSO, extract, ethyl acetate fraction 5% (ET5%), 10% (ET10%), 20% (ET20%), 40% (ET40 %). Data were analyzed statistically by ANOVA and chemometrically with PCA. The inhibition zone for S.aureus bacteria in each sample is 26.69; 1.40; 23.32;2.82; 6.23; 11.11; 20.15 mm, respectively, E.coli is 26.65;0.63;22.65;3.61;7.11;11.44;21.15 mm respectively, P. aeruginosa is 27.40; 0.00; 23.23; 2.74;7.03;11.69;21.36 mm respectively. Percent inhibition of extract, ET5%, ET10%, ET20%, ET40% on S. aureus bacteria is 82.16; 5.31; 18.12; 36.39; 70.38% respectively, E.coli is 82.67; 11.13; 24.31; 40.56; 76.99% respectively, P. aeruginosa 84.85; 10.01; 25.65; 42.68; 77.98% respectively. Extract and ethyl acetate fraction have significant potential as antibacterial (p<0.05). The results of PCA chemometric analysis showed that the extract and ET40% had similar inhibition zone area to the positive control ciprofloxacin. The extract and the ethyl acetate fraction 40% are promising for development as antibacterials.\nKeywords: Sansevieria trifasciata Prain., chemometric, bacterial","PeriodicalId":473028,"journal":{"name":"Jurnal Sains dan Kesehatan","volume":"21 2","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jurnal Sains dan Kesehatan","FirstCategoryId":"0","ListUrlMain":"https://doi.org/10.25026/jsk.v6i1.2087","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Exploration the antibacterial activity of S.trifasciata Prain. is still limited, therefore this study aims to assess the antibacterial activity of extracts and ethyl acetate fractions of Sansevieria trifasciata Prain. The S.trifasciata leaves was macerated with ethanol 96%, then fractionated using the trituration method with ethyl acetate. The treatment group was divided into positive control group (PC) using ciprofloxacin, negative control (NC) using DMSO, extract, ethyl acetate fraction 5% (ET5%), 10% (ET10%), 20% (ET20%), 40% (ET40 %). Data were analyzed statistically by ANOVA and chemometrically with PCA. The inhibition zone for S.aureus bacteria in each sample is 26.69; 1.40; 23.32;2.82; 6.23; 11.11; 20.15 mm, respectively, E.coli is 26.65;0.63;22.65;3.61;7.11;11.44;21.15 mm respectively, P. aeruginosa is 27.40; 0.00; 23.23; 2.74;7.03;11.69;21.36 mm respectively. Percent inhibition of extract, ET5%, ET10%, ET20%, ET40% on S. aureus bacteria is 82.16; 5.31; 18.12; 36.39; 70.38% respectively, E.coli is 82.67; 11.13; 24.31; 40.56; 76.99% respectively, P. aeruginosa 84.85; 10.01; 25.65; 42.68; 77.98% respectively. Extract and ethyl acetate fraction have significant potential as antibacterial (p<0.05). The results of PCA chemometric analysis showed that the extract and ET40% had similar inhibition zone area to the positive control ciprofloxacin. The extract and the ethyl acetate fraction 40% are promising for development as antibacterials. Keywords: Sansevieria trifasciata Prain., chemometric, bacterial

查看原文本刊更多论文

体外方法：Sansevieria trifasciata Prain.叶片的抗菌活性

对 S.trifasciata Prain 抗菌活性的探索仍然有限，因此本研究旨在评估 S.trifasciata Prain 的提取物和乙酸乙酯馏分的抗菌活性。用 96% 的乙醇浸泡 S.trifasciata 叶片，然后用乙酸乙酯三重法进行馏分。处理组分为使用环丙沙星的阳性对照组（PC）、使用二甲基亚砜的阴性对照组（NC）、提取物、乙酸乙酯馏分 5% (ET5%)、10% (ET10%)、20% (ET20%)、40% (ET40%)。数据采用方差分析和 PCA 化学计量学方法进行统计分析。各样品对金黄色葡萄球菌的抑菌区分别为 26.69; 1.40; 23.32; 2.82; 6.23; 11.11; 20.15 mm，对大肠杆菌的抑菌区分别为 26.65; 0.63; 22.65; 3.61; 7.11; 11.44; 21.15 mm，对绿脓杆菌的抑菌区分别为 27.40; 0.00; 23.23; 2.74; 7.03; 11.69; 21.36 mm。提取物、ET5%、ET10%、ET20%、ET40% 对金黄色葡萄球菌的抑菌率分别为 82.16%；5.31%；18.12%；36.39%；70.38%；对大肠杆菌的抑菌率分别为 82.67%；11.13%；24.31%；40.56%；76.99%；对铜绿假单胞菌的抑菌率分别为 84.85%；10.01%；25.65%；42.68%；77.98%。提取物和乙酸乙酯馏分具有显著的抗菌潜力（p<0.05）。PCA 化学计量分析结果表明，提取物和 ET40% 的抑菌区面积与阳性对照环丙沙星相似。该提取物和乙酸乙酯馏分40%有望作为抗菌药开发：三叶草化学计量学细菌

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Jurnal Sains dan Kesehatan

自引率

0.00%

发文量