EFFECT OF LOW LEVEL LASER OF 650NM ON VIALBILITY OF PREVOTELLA INTERMEDIAWITH AND WITHOUT PHOTOSENSITISERS: AN IN VITRO STUDY

M.M Dayakar, Mohammed Nihal Abbas Majeed, Apoorva Gurulingappa
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Abstract

Background: Prevotella intermedia is commonly associated with various forms of periodontal disease. Conventional treatment involves a combination of mechanical therapy and systemic antibiotics administration, which comes with its own set of challenges and disadvantages. An alternative treatment modality is low-level laser therapy with and without photosensitizers. Purpose: This study aimed to evaluate the effectiveness of low-level laser therapy with and without the use of photosensitizers, specifically Methylene blue and Toluidine blue, on the viability of Prevotella intermedia. Methods: The study utilized in vitro experiments to assess the impact of low-level laser therapy alone and in combination with photosensitizers on the viability of Prevotella intermedia. Results: The findings indicate that Methylene blue, when combined with low-level laser therapy, exhibits bactericidal effects similar to or greater than low-level laser therapy alone on Prevotella intermedia. Additionally, the use of Toluidine blue as a photosensitizer in combination with low-level laser therapy also led to a reduction in the viability of Prevotella intermedia, although it was not as effective as Methylene blue. Conclusion: Low-level laser therapy, particularly when combined with Methylene blue as a photosensitizer, shows promise as a potential treatment modality for controlling Prevotella intermedia in periodontal diseases.
波长为 650nm 的低强度激光对含光敏剂和不含光敏剂的间盘尾丝菌生命力的影响:体外研究
背景:中前孢子菌通常与各种形式的牙周病有关。传统的治疗方法包括机械治疗和全身使用抗生素,但这两种方法都有各自的挑战和缺点。另一种治疗方法是使用或不使用光敏剂的低强度激光疗法。目的:本研究旨在评估使用或不使用光敏剂(特别是亚甲蓝和甲苯胺蓝)的低强度激光疗法对中间前牙菌活力的影响。研究方法该研究利用体外实验来评估低强度激光疗法单独使用或与光敏剂联合使用对中间普雷沃氏菌活力的影响。结果显示研究结果表明,亚甲基蓝与低强度激光疗法相结合,对中间普雷沃氏菌的杀菌效果与单独使用低强度激光疗法相似或更强。此外,将甲苯胺蓝作为光敏剂与低强度激光疗法结合使用,也能降低中间普雷沃氏菌的存活率,但效果不如甲苯胺蓝。结论低强度激光疗法,尤其是结合亚甲蓝作为光敏剂时,有望成为控制牙周病中间普雷沃氏菌的一种潜在治疗方式。
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