Capsosiphon fulvescens suppresses LPS-stimulated inflammatory responses by suppressing TLR4/NF-κB activation in RAW264.7 murine macrophages

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-01 DOI:10.4103/apjtb.apjtb_865_23

S. Ji, EunJin Bang, Hyun Hwangbo, Min Yeong Kim, Da Hye Kim, S. Hong, S. Park, C. Kwon, Gi-Young Kim, You-Jin Jeon, Suengmok Cho, Yung Hyun Choi

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Abstract

To evaluate the effects of Capsosiphon fulvescens (C. fulvescens) ethanolic extract on inflammation in lipopolysaccharide (LPS)-induced RAW296.7 macrophages. The protective effects of C. fulvescens ethanolic extract on LPS-induced inflammation in RAW264.7 macrophages were assessed using biochemical analysis, including enzyme-linked immunosorbent assay, quantitative reverse transcription-polymerase chain reaction, and Western blot analysis. To examine reactive oxygen species (ROS) production, flow cytometry analysis, and immunofluorescence staining were used. Furthermore, the modulatory effect of C. fulvescens ethanolic extract on NF-κB activation was investigated. C. fulvescens ethanolic extract significantly attenuated LPS-induced levels of pro-inflammatory cytokines and notably reduced the secretion and mRNA levels of LPS-mediated matrix metalloproteinases. In addition, C. fulvescens ethanolic extract decreased ROS production and suppressed the TLR4/NF-κB signaling pathway. C. fulvescens ethanolic extract alleviates inflammation as well as oxidative stress by modulating the TLR4/NF-κB signaling in LPS-induced RAW264.7 macrophages. C. fulvescens can be used as a potential therapeutic agent to suppress inflammation and oxidative stress-associated diseases.

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辣椒素通过抑制 RAW264.7 小鼠巨噬细胞中 TLR4/NF-κB 的激活来抑制 LPS 刺激的炎症反应

目的：评估白头翁乙醇提取物对脂多糖（LPS）诱导的 RAW296.7 巨噬细胞炎症的影响。通过生化分析，包括酶联免疫吸附试验、定量反转录聚合酶链反应和 Western 印迹分析，评估了白头翁乙醇提取物对 LPS 诱导的 RAW296.7 巨噬细胞炎症的保护作用。为了检测活性氧（ROS）的产生，使用了流式细胞仪分析和免疫荧光染色。此外，还研究了白千层乙醇提取物对 NF-κB 活化的调节作用。黄花菜乙醇提取物能明显减轻 LPS 诱导的促炎细胞因子水平，并显著降低 LPS 介导的基质金属蛋白酶的分泌和 mRNA 水平。此外，胡黄连乙醇提取物还能减少 ROS 的产生，抑制 TLR4/NF-κB 信号通路。黄花菜乙醇提取物通过调节 LPS 诱导的 RAW264.7 巨噬细胞中的 TLR4/NF-κB 信号传导，缓解了炎症和氧化应激。黄花菜可作为一种潜在的治疗药物，用于抑制炎症和氧化应激相关疾病。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.