Icariin ameliorates viral myocarditis by inhibiting TLR4-mediated ferroptosis

IF 1.7 4区医学 Q3 TROPICAL MEDICINE

Asian Pacific journal of tropical biomedicine Pub Date : 2024-03-01 DOI:10.4103/apjtb.apjtb_588_23

Wei Luo, Yi Lu, Jun Deng, Peng Liu, Yan Huang, Wan-Xi Liu, Chun-Li Huang

{"title":"Icariin ameliorates viral myocarditis by inhibiting TLR4-mediated ferroptosis","authors":"Wei Luo, Yi Lu, Jun Deng, Peng Liu, Yan Huang, Wan-Xi Liu, Chun-Li Huang","doi":"10.4103/apjtb.apjtb_588_23","DOIUrl":null,"url":null,"abstract":"\n \n To explore the mechanism by which icariin alleviates viral myocarditis.\n \n \n \n CVB3-induced cardiomyocytes were used as an in vitro model of viral myocarditis to assess the effects of icariin treatment on cell viability, inflammation, and apoptosis. Moreover, the effects of icariin on ferroptosis and TLR4 signaling were assessed. After AC16 cells were transfected with TLR4 overexpression plasmids, the role of TLR4 in mediating the regulatory effect of icariin in viral myocarditis was investigated.\n \n \n \n Icariin significantly elevated cell viability and reduced inflammatory factors TNF-α, IL-1β, IL-6, and IL-18. Flow cytometry revealed that icariin decreased apoptosis rate, and the protein expression of Bax and cleaved caspase 3 and 9 in CVB3-induced cardiomyocytes. Additionally, it suppressed ferroptosis including lipid peroxidation and ferrous ion, as well as the TLR4 signaling. However, TLR4 overexpression abrogated the modulatory effects of icariin.\n \n \n \n Icariin mitigates CVB3-induced myocardial injury by inhibiting TLR4-mediated ferroptosis. Further animal study is needed to verify its efficacy.\n","PeriodicalId":8560,"journal":{"name":"Asian Pacific journal of tropical biomedicine","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Asian Pacific journal of tropical biomedicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4103/apjtb.apjtb_588_23","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"TROPICAL MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

To explore the mechanism by which icariin alleviates viral myocarditis. CVB3-induced cardiomyocytes were used as an in vitro model of viral myocarditis to assess the effects of icariin treatment on cell viability, inflammation, and apoptosis. Moreover, the effects of icariin on ferroptosis and TLR4 signaling were assessed. After AC16 cells were transfected with TLR4 overexpression plasmids, the role of TLR4 in mediating the regulatory effect of icariin in viral myocarditis was investigated. Icariin significantly elevated cell viability and reduced inflammatory factors TNF-α, IL-1β, IL-6, and IL-18. Flow cytometry revealed that icariin decreased apoptosis rate, and the protein expression of Bax and cleaved caspase 3 and 9 in CVB3-induced cardiomyocytes. Additionally, it suppressed ferroptosis including lipid peroxidation and ferrous ion, as well as the TLR4 signaling. However, TLR4 overexpression abrogated the modulatory effects of icariin. Icariin mitigates CVB3-induced myocardial injury by inhibiting TLR4-mediated ferroptosis. Further animal study is needed to verify its efficacy.

查看原文本刊更多论文

淫羊藿苷通过抑制 TLR4 介导的铁变态反应改善病毒性心肌炎

探索冰片素缓解病毒性心肌炎的机制。以 CVB3 诱导的心肌细胞作为病毒性心肌炎的体外模型，评估冰片苷处理对细胞活力、炎症和凋亡的影响。此外，还评估了冰片花素对铁变态反应和 TLR4 信号转导的影响。用 TLR4 过表达质粒转染 AC16 细胞后，研究了 TLR4 在病毒性心肌炎中介导冰片素调节作用的作用。水淫羊藿苷明显提高了细胞活力，降低了炎症因子 TNF-α、IL-1β、IL-6 和 IL-18。流式细胞术显示，水淫羊藿苷降低了 CVB3 诱导的心肌细胞的凋亡率，以及 Bax 和裂解的 Caspase 3 和 9 的蛋白表达。此外，它还能抑制铁变态反应，包括脂质过氧化和亚铁离子，以及 TLR4 信号传导。然而，TLR4 的过表达会减弱淫羊藿苷的调节作用。淫羊藿苷通过抑制 TLR4 介导的铁肽化来减轻 CVB3 诱导的心肌损伤。要验证其疗效，还需要进一步的动物实验。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Asian Pacific journal of tropical biomedicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)

CiteScore

3.10

自引率

11.80%

发文量

2056

审稿时长

4 weeks

期刊介绍： The journal will cover technical and clinical studies related to health, ethical and social issues in field of biology, bacteriology, biochemistry, biotechnology, cell biology, environmental biology, microbiology, medical microbiology, pharmacology, physiology, pathology, immunology, virology, toxicology, epidemiology, vaccinology, hematology, histopathology, cytology, genetics and tropical agriculture. Articles with clinical interest and implications will be given preference.