A Two-Stage Transferred Cold Atmospheric Plasma as a Unique Therapeutic Strategy for Targeting Colon Cancer Stem Cells

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Abolfazl Soulat, Taghi mohsenpour, Leila Roshangar, H. Naghshara
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引用次数: 0

Abstract

The study examines the induction of apoptosis in colon cancer stem cells (CCSCs) within a 3D culture setting, employing an innovative cold atmospheric plasma (CAP) transmission method known as TS-TCAP. TS-TCAP is a partially or fully ionized non-thermal gaseous mixture that comprises photons, charged and neutral particles, and free radicals, which has gained traction in biomedical applications such as cancer therapy. TS-TCAP impacts CCSCs via a continuous, two-step transport process, facilitating the efficient delivery of reactive oxygen and nitrogen species (RONS). The key cellular factors of CCSCs impacted by TS-TCAP treatment, encompassing the secretion and expression levels of IL-6 and IL-8, apoptotic cell count, and expression of BAX, BCL-2, and KI-67 proteins, were evaluated using qrt-ELISA, Annexin V, and qrt-PCR procedures, respectively. The outcomes of CCSCs treatment with TS-TCAP reveal a notable rise in the number of apoptotic cells (p value <0.0001), diminished secretion, and gene expression of IL-6 and IL-8 (p-value < 0.0001), accompanied by favorable alterations in BCL-2 and BAX gene expression (p value <0.0001). Additionally, a notable decrease in KI-67 expression was observed, correlating with a reduction in CCSCs proliferation (p value <0.0001). As well, this study underscores the anti-cancer potential of TS-TCAP, showcasing its efficacy in reducing CCSCs survival rates. However, further pre-clinical and clinical trials are necessary to evaluate CAP's efficacy, safety, and potential synergistic effects with other therapies thoroughly. Overall, TS-TCAP presents a promising alternative for CCSCs treatment, pending further investigation and refinement.
两级转移冷大气等离子体作为针对结肠癌干细胞的独特治疗策略
这项研究采用一种名为 TS-TCAP 的创新型冷大气等离子体 (CAP) 透射法,研究了在三维培养环境中诱导结肠癌干细胞 (CCSC) 细胞凋亡的问题。TS-TCAP是一种部分或完全电离的非热性气态混合物,由光子、带电粒子、中性粒子和自由基组成,已在癌症治疗等生物医学应用中得到广泛应用。TS-TCAP 通过连续的两步传输过程影响 CCSCs,促进活性氧和氮物种(RONS)的有效传输。使用 qrt-ELISA、Annexin V 和 qrt-PCR 程序分别评估了经 TS-TCAP 处理的 CCSCs 的关键细胞因子,包括 IL-6 和 IL-8 的分泌和表达水平、凋亡细胞数量以及 BAX、BCL-2 和 KI-67 蛋白的表达。用TS-TCAP处理CCSCs的结果显示,凋亡细胞数量显著增加(p值<0.0001),IL-6和IL-8的分泌和基因表达减少(p值<0.0001),同时BCL-2和BAX基因表达也发生了有利的变化(p值<0.0001)。此外,还观察到 KI-67 表达明显减少,与 CCSCs 增殖减少相关(p 值 <0.0001)。这项研究还强调了 TS-TCAP 的抗癌潜力,展示了它在降低 CCSCs 存活率方面的功效。不过,还需要进一步的临床前和临床试验来全面评估 CAP 的疗效、安全性以及与其他疗法的潜在协同作用。总之,TS-TCAP是一种治疗CCSCs的有前途的替代疗法,有待进一步研究和完善。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
期刊介绍: ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric. Indexed/​Abstracted: Web of Science SCIE Scopus CAS INSPEC Portico
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