Immuno-Haematological Indices Stabilizing Attributes of Co-Enzyme Q10 Supplementation in Thioacetamide Poisoned Wistar Rats

Abstract

Coenzyme Q10 (CoQ10 or Co_Q10) as a known endogenous compound has been linked to some therapeutic functions, thus, the need for further exploration in more dysfunctional biological scenarios. This present study, thus, evaluated the stabilizing attributes of Co-enzyme Q10 supplementation in thioacetamide (TAA) altered immuno-haematological system in Wistar rats. One hundred and twenty (120) male Wistar rats weighing 250 and 280g were used for the study and were randomly separated into four sub-groups of 30 rats per treatment interval of week 3, 6, 9 and 12. In the individual weeks, the rats were further grouped into six different groups of five rats each. The groups included Group 1-control and received 1ml of normal saline (intraperitoneally―ip), Group 2 received only 200 mg/kg TAA (ip) twice weekly, Group 3 received 5mg/kg Co_Q10, daily per oral (po), Group 4 received 10mg/kg Co_Q10 daily (po), Group 5 received 200 mg/kg TAA (ip) twice weekly and 5mg/kg Co_Q10 (po) daily and Group 6 received 200 mg/kg TAA (ip) twice weekly and 10mg/kg Co_Q10 (po) daily. After weeks 3, 6, 9 and 12 of treatments, blood samples were collected and instantly transferred into well labeled blood sample bottles with anticoagulant. After appropriate laboratory and statistical analyses, the quantitative results were obtained. The result showed that following TAA toxicity, the levels of white blood cells (WBC) were significantly (P<0.05) reduced; meanwhile, those of macrophage and tissue necrotic factor alpha (TNFα) were remarkably (P<0.05) increased. However, upon the supplementations with Co-Q10 in the respective weeks (3, 6, 9 and 12) of treatment, all the aforementioned deranged values of WBC, macrophages and TNFα were significantly (P<0.05) stabilized/normalized (i.e. brought to similar range of the normal untreated rats). Therefore, Co-Q10 supplementation may be of beneficial therapeutic value in particular haematological dysfunctional scenarios.