Development and validation of an analytical method for acetamiprid determination in plant protection products using HPLC-DAD

Abstract

Plant protection products (PPP), crucial for agricultural production, are experiencing increased global demand, particularly with the growing need for food production. To meet this demand, robust analytical methods are essential for confirming the presence and determining active substance concentrations in PPP. This study introduces an analytical method utilizing high-performance liquid chromatography with a diode array detector (HPLC-DAD) for determination of acetamiprid in water-soluble powder formulations. The method, validated according to SANCO/3030/99 rev.5, demonstrated exhibited adequate accuracy and precision, with repeatability expressed as the ratio of the standard deviation (% RSD) to the relative standard deviation (% RSDr) being lower than 1. Recoveries for the active substance at concentrations above 10% ranged from 97% to 103%. The developed method is also characterized by suitable linearity, confirmed by a correlation coefficient >0.99. Specific chromatographic profiles were generated, and acetamiprid content in 180 formulations was analyzed, including reference products. The developed method aligns with “green chemistry” principles, minimizing solvent use and emphasizing energy efficiency. Overall, it offers a comprehensive approach for qualitative and quantitative analysis, ensuring the reliability of PPP quality control.