Platelet Additive Solutions SSP+ and T-PAS+ Are Interchangeable for Platelet Concentrate Storage despite Differences in Composition and Plasticizer

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-26 DOI:10.1159/000538003

Britt Van Aelst, Karen Bens, B. Sabot, Sarah Vertongen, H. Feys, V. Compernolle, Katrijn R. Six

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引用次数: 0

Abstract

Introduction: Platelet additive solutions support ex vivo storage of platelet concentrates used for transfusion. The composition of platelet additive solutions within one generation (i.e., PAS-E) is similar but not identical. Additionally, the platelet additive solution storage bag may contain different plasticizers. This study compares the effect of two PAS-E solutions (SSP+ vs. T-PAS+, stored in a DEHP-containing and DEHP-free bag, respectively) to investigate if both additive solutions are interchangeable for platelet concentrate storage. Methods: Platelet concentrates stored in plasma supplemented with SSP+ or T-PAS+ were compared by using a pool-and-split design. Platelet metabolism was investigated using a blood gas analyzer. The degree of platelet storage lesion was determined by flow cytometry to measure granule release and phosphatidylserine scrambling. Results: The quality of platelet concentrates stored in either SSP+ or T-PAS+ is acceptable as pH decreased only slightly as a function of time. PH remained above 7.2 on exiration day +1 (day 6), which is far above the minimal criterion of 6.4. Platelet storage lesion was comparable between the two study groups with only limited α-granule release and phosphatidylserine surface expression in both groups after storage for 5 days, p = 0.547 and p = 0.825, respectively. Conclusion: This study supports a safe switch between SSP+ and T-PAS+ storage solutions for platelet concentrates despite slight differences in storage solution composition and DEHP content.

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尽管成分和增塑剂不同，血小板添加剂溶液 SSP+ 和 T-PAS+ 仍可互换用于血小板浓缩液的储存

简介血小板添加剂溶液支持用于输血的血小板浓缩物的体外储存。同一代（即 PAS-E）血小板添加剂溶液的成分相似，但并不完全相同。此外，血小板添加剂溶液储存袋可能含有不同的增塑剂。本研究比较了两种 PAS-E 溶液（SSP+ 与 T-PAS+，分别储存在含 DEHP 和不含 DEHP 的袋子中）的效果，以调查这两种添加剂溶液在血小板浓缩液储存中是否可以互换。方法：采用集合-分割设计，对储存在补充了 SSP+ 或 T-PAS+ 的血浆中的血小板浓缩物进行比较。使用血气分析仪调查血小板的新陈代谢。通过流式细胞术测量颗粒释放和磷脂酰丝氨酸扰乱，确定血小板储存病变的程度。结果储存在 SSP+ 或 T-PAS+ 中的血小板浓缩物的质量是可以接受的，因为 pH 值随着时间的推移仅略有下降。PH 值在出库第 +1 天（第 6 天）仍保持在 7.2 以上，远高于 6.4 的最低标准。两个研究组的血小板贮存病变情况相当，贮存 5 天后，两组都只有有限的 α 颗粒释放和磷脂酰丝氨酸表面表达，分别为 p = 0.547 和 p = 0.825。结论尽管血小板浓缩物的储存溶液成分和 DEHP 含量略有不同，但本研究支持在 SSP+ 和 T-PAS+ 储存溶液之间进行安全转换。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.