IMPROVEMENT OF TECHNIQUES FOR TRANSPLANTING HEALTHY IN VITRO GRAPE PLANTS IN SOIL CULTURE CONDITIONS

Ekaterina Viktorovna Lopatkina, A. Rebrov
{"title":"IMPROVEMENT OF TECHNIQUES FOR TRANSPLANTING HEALTHY IN VITRO GRAPE PLANTS IN SOIL CULTURE CONDITIONS","authors":"Ekaterina Viktorovna Lopatkina, A. Rebrov","doi":"10.30679/2219-5335-2024-2-86-103-114","DOIUrl":null,"url":null,"abstract":"The article discusses techniques for improving the properties of a nutrient substrate used for planting healthy in vitro grape plants. The research was carried out on the basis of the laboratory of biotechnology ARRIV&W – branch of FSBSI FRARC on an interspecific hybrid Krasnostop Carpi. The purpuse of the study is to improve the ways of adapting healthy in vitro plants to non–sterile conditions. With microclonal reproduction of grapes, death most often occurs when transferring test tube plants to non-sterile conditions. The selection of the composition of the soil substrate and the improvement of its water-physical and biological properties helps to reduce the risk of plant death. In our studies, peat, garden soil and river sand (1:1:1) were used as part of the substrate, to which “Aquasin hydrogel” and “Trichoderma Veride” endomycorrhizal preparation containing mycelium and spores of Trichoderma viride fungi, strain 471, were added. The plants were planted in a 500 ml container, had been adapted to non-sterile conditions and had been grown for 90 days on racks of accelerated plant cultivation (RAPC). In the process, the indicators of the preservation of initial plants, their growth, development and spread of the root system were noted. As a result, it can be concluded that the combined use of hydrogel and mycorrhiza ensures the best survival and preservation of plants of the Krasnostop Carpi variety. The mycorrhizal preparation promotes better development of the leaf surface, and the addition of hydrogel ensures better development of the root system, which will provide plants with better survival in open ground conditions of the basic mother plantation. Key words: HEALTHY IN VITRO GRAPES, HYDROGEL, MYCORRHIZA, SUBSTRATE, ADAPTATION TO NON-STERILE CONDITIONS","PeriodicalId":504482,"journal":{"name":"Fruit growing and viticulture of South Russia","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fruit growing and viticulture of South Russia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.30679/2219-5335-2024-2-86-103-114","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

The article discusses techniques for improving the properties of a nutrient substrate used for planting healthy in vitro grape plants. The research was carried out on the basis of the laboratory of biotechnology ARRIV&W – branch of FSBSI FRARC on an interspecific hybrid Krasnostop Carpi. The purpuse of the study is to improve the ways of adapting healthy in vitro plants to non–sterile conditions. With microclonal reproduction of grapes, death most often occurs when transferring test tube plants to non-sterile conditions. The selection of the composition of the soil substrate and the improvement of its water-physical and biological properties helps to reduce the risk of plant death. In our studies, peat, garden soil and river sand (1:1:1) were used as part of the substrate, to which “Aquasin hydrogel” and “Trichoderma Veride” endomycorrhizal preparation containing mycelium and spores of Trichoderma viride fungi, strain 471, were added. The plants were planted in a 500 ml container, had been adapted to non-sterile conditions and had been grown for 90 days on racks of accelerated plant cultivation (RAPC). In the process, the indicators of the preservation of initial plants, their growth, development and spread of the root system were noted. As a result, it can be concluded that the combined use of hydrogel and mycorrhiza ensures the best survival and preservation of plants of the Krasnostop Carpi variety. The mycorrhizal preparation promotes better development of the leaf surface, and the addition of hydrogel ensures better development of the root system, which will provide plants with better survival in open ground conditions of the basic mother plantation. Key words: HEALTHY IN VITRO GRAPES, HYDROGEL, MYCORRHIZA, SUBSTRATE, ADAPTATION TO NON-STERILE CONDITIONS
改进在土壤培养条件下移植健康试管葡萄植株的技术
文章讨论了改善用于种植健康试管葡萄植株的营养基质特性的技术。该研究是在FSBSI FRARC分部生物技术实验室(ARRIV&W)的基础上进行的,研究对象是种间杂交品种 "卡皮"(Krasnostop Carpi)。研究的目的是改进使健康的试管植物适应非无菌条件的方法。在葡萄的微克隆繁殖中,将试管植株转移到非无菌条件下最常出现的情况是死亡。选择土壤基质的成分并改善其水物理和生物特性有助于降低植物死亡的风险。在我们的研究中,泥炭、园土和河沙(1:1:1)被用作基质的一部分,并加入了 "Aquasin 水凝胶 "和 "Trichoderma Veride "内生菌根制剂,其中含有病毒毛霉菌株 471 的菌丝和孢子。植物种植在 500 毫升的容器中,已适应非无菌条件,并在植物加速栽培架(RAPC)上生长了 90 天。在此过程中,我们注意到了初始植物的保存、生长、发育和根系扩展等指标。因此,可以得出结论:水凝胶和菌根的结合使用确保了 Krasnostop Carpi 品种植物的最佳存活和保存。菌根制剂能促进叶面更好地发育,而水凝胶的添加则能确保根系更好地发育,从而使植物在基本母本种植园的露地条件下更好地存活。关键字健康离体葡萄、水凝胶、菌根、基质、适应非无菌条件
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信