Haplotype Affiliation of Varroa destructor in Selected Locations from Poland and Ukraine

IF 0.7 4区农林科学 Q4 ENTOMOLOGY

Journal of Apicultural Science Pub Date : 2024-03-29 DOI:10.2478/jas-2024-0001

Piotr Dziechciarz, Marcin Domaciuk, M. Iller, Stefan Kerek, Grzegorz Borsuk

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Abstract

The virulence patterns observed in viruses transmitted by Varroa destructor implied a potential association of these honey bee pests in Poland with the Korean haplotype. Nevertheless, it is noteworthy that this hypothesis lacked direct verification. Therefore, the aim of the study was to determine the haplotype affiliation of V. destructor mites infesting bees in Poland and Ukraine. Adult females were collected from drone brood from honey bee colonies maintained in the apiary at the University of Life Sciences in Lublin, Poland, and the apiary in Synevyr National Park in Ukraine. The haplotype affiliation was determined through the sequencing of the cytochrome oxidase subunit I gene (CO I; 929 bp) fragment. Based on the phylogenetic comparison with 84% bootstrap support, the V. destructor females from both locations were confirmed to represent three haplotypes: Korean 1 (AmK1-1), Korean 2 (AmK1-2), and Chinese 4 (AmK1-4). They are the most virulent types of this parasite worldwide and in Poland. Concurrently, the present study confirms that the Synevyr National Park in Ukraine is isolated from alien bee subspecies, but is not free from alien parasites.

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波兰和乌克兰部分地区破坏者 Varroa 的单倍型隶属关系

在破坏者 Varroa 传播的病毒中观察到的毒力模式意味着波兰的这些蜜蜂害虫可能与韩国单倍型有关。然而，值得注意的是，这一假设缺乏直接验证。因此，本研究的目的是确定波兰和乌克兰蜜蜂所感染的破坏螨的单倍型归属。研究人员从波兰卢布林生命科学大学养蜂场和乌克兰 Synevyr 国家公园养蜂场的蜜蜂蜂群中采集了雌蜂。通过对细胞色素氧化酶亚基 I 基因（CO I；929 bp）片段进行测序，确定了单倍型的隶属关系。根据系统进化比较（84%的引导支持率），两地的毁灭蚁雌性均被证实代表三种单倍型：韩国 1 型（AmK1-1）、韩国 2 型（AmK1-2）和中国 4 型（AmK1-4）。它们是这种寄生虫在全球和波兰毒性最强的类型。同时，本研究证实，乌克兰的 Synevyr 国家公园与外来蜜蜂亚种隔离，但并非没有外来寄生虫。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Apicultural Science ENTOMOLOGY-

CiteScore

1.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Apicultural Science is a scientific, English-language journal that publishes both original research articles and review papers covering all aspects of the life of bees (superfamily Apoidea) and broadly defined apiculture. The main subject areas include: -bee biology- bee genetics- bee breeding- pathology and toxicology- pollination and bee botany- bee products- management, technologies, and economy- solitary bees and bumblebees