Phytochemical Characteristic Analysis and Biological Activity for Capparis spinosa L. Fruit extract

Salma Neamah, I. Falih, Salim Albukhaty, Sasikumar P
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Abstract

Alternative medicine and herbal treatment are among the methods inherited by the people of the Middle East. In the current study, we evaluated the chemical composition of the fruit of Capparis spinosa L. ( C. spinosea L.), as well as the antibacterial, and potential inhibitory effects of the dehydrogenase enzyme (LDH). The presence of the active chemicals in the extract was confirmed by phytochemical screening and characterization techniques such as gas chromatography-mass spectrometry analysis (GC-MS), ultraviolet (UV-Vis) spectroscopy, and infrared spectroscopy (FTIR). The outcomes demonstrated the presence of alkaloids, flavonoids, polyphenols, tannins, and vitamins in the aqueous extract. Moreover, the extract exhibited anti-bacterial activity, especially against gram-negative bacteria. In contrast, the concentrations of the aqueous extract possessed the activity of inhibiting the dehydrogenase enzyme at a concentration of 0.13 mmo l/L. The study concluded that C.spinosea L. fruit aqueous extract contains biologically active compounds that could be used in the inhibition processes of both antibacterial and as well as inhibitors of lactate dehydrogenase (LDH).
Capparis spinosa L. 果实提取物的植物化学特性分析和生物活性
替代医学和草药治疗是中东人民传承下来的方法之一。在本研究中,我们评估了 Capparis spinosa L. (C. spinosea L.)果实的化学成分,以及抗菌和潜在的脱氢酶(LDH)抑制作用。通过植物化学筛选和表征技术,如气相色谱-质谱分析法(GC-MS)、紫外-可见光谱法(UV-Vis)和红外光谱法(FTIR),确认了提取物中活性化学物质的存在。结果表明,水提取物中含有生物碱、黄酮类、多酚类、单宁酸和维生素。此外,提取物还具有抗菌活性,尤其是对革兰氏阴性菌。相比之下,浓度为 0.13 mmo l/L 的水提取物具有抑制脱氢酶的活性。研究得出结论,C.spinosea L. 果实水提取物含有生物活性化合物,可用于抗菌和抑制乳酸脱氢酶(LDH)。
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