Effect of Chitosan Nanoparticles on Growth, Swarming Motility, and Biofilm Formation in Proteus Mirabilis Isolated from Urinary Tract Infections

Abstract

Proteus mirabilis is the third most prevalent cause of urinary tract infection (UTI), the goal of this study was to see how chitosan nanoparticles (CS-NPs) affected on growth, swarming motility and biofilm formation of Proteus mirabilis , after isolation from patient infected with urinary tract infection and identified with routine methods and confirmed using API system. Minimum inhibitory concentration (MIC) of chitosan nanoparticles (CS-NPs) against the clinical strain of Proteus mirabilis was identified. The sub-MIC (CS-NPS) was used to evaluate their inhibitory effect on swarming motility and forming of biofilm. The results showed that the chitosan nanoparticles caused a remarkable inhibition of the two important virulence factors swarming motility and biofilm formation as compared with the control. As well as swarming motility's mean diameter was reduced from 8.5 to 0.5 cm. The clinical strain of Proteus mirabilis developed into a moderate biofilm producer after treatment, with a mean percentage rate of biofilm inhibition of 58%.