Synthesis, Characterization and Study Biological Activity of Substituted 4-Amino -3,5-Bis (2,4-dichloro phenoxy)-1,2,4-Triazole

Abstract

In this paper, the ester (2) was prepared by reacting 2,4-dichlorophenoxy acetic acid with methyl alcohol and using concentrated sulfuric acid as a catalyst in this reaction, which, when reacted with hydrazine hydrate, obtained acid hydrazide. (3), under reflux conditions with dimethyl sulfoxide and at solvent temperature, the compound 4-amino-3,5-bis-(2,4-dichlorophenoxy)-1,2,4-triazole was obtained (4). It was obtained and used to prepare a series of new Schiff bases (5a-e) through their reaction with different substitutes of benzaldehyde. The completeness and purity of all the prepared compounds were confirmed by thin-layer chromatography (TLC) and showed some physical and spectroscopic properties, namely (FT -IR 1H-NMR and 13C-NMR). In addition, the biological activity of the prepared compounds was studied, and their effectiveness against different types of bacteria such as Bacillus, Escherichia coli , and Staphylococcus aureus was discussed. Studies of mainly fungi and bacteria have shown good efficacy against the mentioned species using disc diffusion technology.