Assessment of the Impact of Preanalytical DNA Integrity on the Genome Data Quality.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-01 Epub Date: 2024-04-01 DOI:10.1089/bio.2023.0050

Sung-Mi Shim, Meehee Lee, Jae-Pil Jeon

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引用次数: 0

Abstract

Many molecular approaches have been employed for the quality control (QC) of biobanked DNA samples. Since 2003, the National Biobank of Korea (NBK) has provided various studies with over half a million quality-controlled genomic DNA samples using conventional agarose gel electrophoresis and spectrophotometry. We assessed the postanalytical genomic data quality of DNA samples (n = 41) with a different range of the DNA quality index such as genomic quality number (GQN) for developing an evidence-based best practice for DNA quality criteria. We examined the quality indices of three different platforms, including single nucleotide polymorphism arrays, methylation arrays, and next-generation sequencing, using the same DNA samples (n = 41) of different quality, ranging from 4.0 to 10.0 values of the GQN. Our data analysis revealed that higher GQN value and/or double-stranded DNA concentration resulted in higher quality genomic data. In addition, all the analyzed DNA samples successfully generated good-quality genomic data. This study provides a guide for the QC of biobanked DNA samples for genomic analysis platforms.

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评估分析前 DNA 完整性对基因组数据质量的影响。

许多分子方法被用于生物库 DNA 样品的质量控制（QC）。自 2003 年以来，韩国国家生物库（NBK）利用传统的琼脂糖凝胶电泳和分光光度法为各种研究提供了超过 50 万份经过质量控制的基因组 DNA 样本。我们用不同范围的 DNA 质量指标（如基因组质量数（GQN））评估了 DNA 样本（n = 41）分析后的基因组数据质量，以制定基于证据的 DNA 质量标准最佳实践。我们使用单核苷酸多态性阵列、甲基化阵列和新一代测序等三种不同平台，使用不同质量的DNA样本（n = 41）（GQN值从4.0到10.0不等）检测了它们的质量指数。我们的数据分析显示，GQN 值和/或双链 DNA 浓度越高，基因组数据的质量越高。此外，所有分析过的 DNA 样本都成功生成了高质量的基因组数据。这项研究为基因组分析平台生物库DNA样本的质量控制提供了指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.