Curcumin Lowers the Accelerated Speed of Epileptogenesis by Traumatic Brain Injury.

Q2 Biochemistry, Genetics and Molecular Biology

Iranian Biomedical Journal Pub Date : 2024-03-01 DOI:10.61186/ibj.3978

Hanieh Jahi, Mansoureh Eslami, Mohammad Sayyah, Fariba Karimzadeh, Melika Alesheikh

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Abstract

Background: Traumatic brain injury or TBI can underlie epilepsy. Prevention of PTE has been of great interest to scientists. Given the antiepileptic, antioxidant and anti-inflammatory activities of curcumin, we examined whether this compound can affect epileptogenesis in rats after TBI.

Methods: Curcumin was injected once a day for two weeks. TBI was induced in the temporal cortex of anesthetized rats using a controlled cortical impact device. One day after TBI, pentylenetetrazole (PTZ), 35 mg/kg, was injected i.p. every other day until manifestation of generalized seizures. The number of PTZ injections was then recorded. Moreover, the extent of cortical and hippocampal IL-1β and glial fibrillary acidic protein (GFAP) expression in the epileptic rats were measured by Western blot analysis.

Results: Curcumin 50 and 150 mg/kg prevented the development of kindling, whereas TBI accelerated the rate of kindling. Curcumin 20 mg/kg prohibited kindling facilitation by TBI, and reduced the expression of IL-1β and GFAP induced by TBI.

Conclusion: Curcumin can stop the acceleration of epileptogenesis after TBI in rats. Inhibiting hippocampal and cortical overexpression of IL-1β and GFAP seems to be involved in this activity.

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姜黄素能降低创伤性脑损伤加速癫痫发生的速度

背景：创伤性脑损伤或创伤性脑损伤可导致癫痫。预防 PTE 一直是科学家们非常关注的问题。鉴于姜黄素具有抗癫痫、抗氧化和抗炎活性，我们研究了这种化合物能否影响创伤性脑损伤后大鼠的癫痫发生：姜黄素每天注射一次，连续两周。使用 CCI 装置诱导麻醉大鼠颞叶皮层发生 TBI。创伤性脑损伤一天后，每隔一天给大鼠静脉注射 35 毫克/千克的 PTZ，直到出现全身性癫痫发作。然后记录 PTZ 注射次数。此外，还通过 Western 印迹分析测定了癫痫大鼠皮质和海马 IL-1β 及 GFAP 的表达程度：结果：姜黄素 50 毫克/公斤和 150 毫克/公斤可阻止点状痫的发生，而创伤性脑损伤会加速点状痫的发生。姜黄素 20 毫克/千克可抑制 TBI 对癫痫发作的促进作用，并减少 TBI 诱导的 IL-1β 和 GFAP 的表达：结论：姜黄素能阻止大鼠创伤性脑损伤后癫痫的加速发生。结论：姜黄素能阻止大鼠创伤性脑损伤后癫痫的加速发生，抑制海马和皮质中IL-1β和GFAP的过度表达似乎与这一作用有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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