Protective Effects of Curcumin and Nanomicelle Curcumin on Chlorpyrifos-induced Oxidative Damage and Inflammation in the Uterus, Ovary and Brain of Rats.

IF 2.2 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Maryam Nazarian, Hamed Aramjoo, Babak Roshanravan, Saeed Samarghandian, Tahereh Farkhondeh
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引用次数: 0

Abstract

Background and aims: Chlorpyrifos (CPF), which is classified as an Organophosphorus Pesticide (OP), has been identified as a toxic agent for the reproductive system due to its capacity to induce oxidative stress and inflammation. Curcumin (CUR) has been reported as a natural antioxidant and anti-inflammatory agent that could combat toxicity in various tissues. This study aims to examine the protective effects of CUR and its nanoformulation against reproductive impairment induced by CPF.

Methods: Forty-eight female Wistar albino rats were randomly allocated to six groups (n=8): control (0.5 mL of corn oil, the solvent for CPF), CPF (10 mg/kg), CPF + CUR 100 mg/kg/day, CPF + CUR 300 mg/kg/day, CPF + nano-micelle curcumin (NMC) 2.5 mg/kg/day, and CPF + NMC 5 mg/kg/day. The experimental treatment was performed for 30 days. Then, brain, ovary and uterus tissues were collected for measuring oxidative stress and inflammatory indices.

Results: MDA, NO, IL-6, and TNF-α concentrations significantly increased in the brain, ovary and uterus of the CPF group versus the control group (p < 0.001). The levels of GSH and SOD in the uterus, ovaries, and brain exhibited a significant decrease in the CPF group compared to the control group (p < 0.05). However, CUR (300 mg/kg) and NMC (5 mg/kg) significantly decreased MDA, NO, TNF-α, and Il-6 and increased SOD and GSH levels in the uterus, ovaries and brain of the CPF-exposed animals versus the CPF-exposed non-treated animals (p < 0.001).

Conclusion: Our findings indicated that CUR and NMC could be effective in alleviating CPFinduced reproductive toxicity.

姜黄素和纳米姜黄素对毒死蜱诱导的大鼠子宫、卵巢和脑氧化损伤和炎症的保护作用
背景和目的:毒死蜱(CPF)被归类为有机磷农药(OP),由于其能够诱发氧化应激和炎症,已被确定为对生殖系统有毒的物质。据报道,姜黄素(CUR)是一种天然的抗氧化剂和抗炎剂,可对抗各种组织中的毒性。本研究旨在探讨姜黄素及其纳米制剂对 CPF 诱导的生殖损伤的保护作用:将 48 只雌性 Wistar 白化大鼠随机分为 6 组(n=8):对照组(0.5 毫升玉米油,即氯化石蜡的溶剂)、氯化石蜡组(10 毫克/千克)、氯化石蜡 + CUR 组(100 毫克/千克/天)、氯化石蜡 + CUR 组(300 毫克/千克/天)、氯化石蜡 + 纳米姜黄素(NMC)组(2.5 毫克/千克/天)和氯化石蜡 + NMC 组(5 毫克/千克/天)。实验疗程为 30 天。然后收集脑组织、卵巢组织和子宫组织,测定氧化应激和炎症指标:结果:与对照组相比,CPF 组脑、卵巢和子宫中的 MDA、NO、IL-6 和 TNF-α 浓度明显升高(P < 0.001)。与对照组相比,CPF 组子宫、卵巢和大脑中的 GSH 和 SOD 水平明显下降(p < 0.05)。然而,CUR(300 毫克/千克)和 NMC(5 毫克/千克)能显著降低暴露于 CPF 的动物与未暴露于 CPF 的动物相比在子宫、卵巢和大脑中的 MDA、NO、TNF-α 和 Il-6,并提高 SOD 和 GSH 水平(p < 0.001):我们的研究结果表明,CUR 和 NMC 可有效缓解氯化石蜡引起的生殖毒性。
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来源期刊
Current pharmaceutical biotechnology
Current pharmaceutical biotechnology 医学-生化与分子生物学
CiteScore
5.60
自引率
3.60%
发文量
203
审稿时长
6 months
期刊介绍: Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal includes timely in-depth reviews, original research articles and letters written by leaders in the field, covering a range of current topics in scientific areas of Pharmaceutical Biotechnology. Invited and unsolicited review articles are welcome. The journal encourages contributions describing research at the interface of drug discovery and pharmacological applications, involving in vitro investigations and pre-clinical or clinical studies. Scientific areas within the scope of the journal include pharmaceutical chemistry, biochemistry and genetics, molecular and cellular biology, and polymer and materials sciences as they relate to pharmaceutical science and biotechnology. In addition, the journal also considers comprehensive studies and research advances pertaining food chemistry with pharmaceutical implication. Areas of interest include: DNA/protein engineering and processing Synthetic biotechnology Omics (genomics, proteomics, metabolomics and systems biology) Therapeutic biotechnology (gene therapy, peptide inhibitors, enzymes) Drug delivery and targeting Nanobiotechnology Molecular pharmaceutics and molecular pharmacology Analytical biotechnology (biosensing, advanced technology for detection of bioanalytes) Pharmacokinetics and pharmacodynamics Applied Microbiology Bioinformatics (computational biopharmaceutics and modeling) Environmental biotechnology Regenerative medicine (stem cells, tissue engineering and biomaterials) Translational immunology (cell therapies, antibody engineering, xenotransplantation) Industrial bioprocesses for drug production and development Biosafety Biotech ethics Special Issues devoted to crucial topics, providing the latest comprehensive information on cutting-edge areas of research and technological advances, are welcome. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.
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