Detection and comparison of neutrophil extracellular traps in tissue samples of peri-implantitis, periodontitis, and healthy patients: A pilot study

IF 3.7 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical Implant Dentistry and Related Research Pub Date : 2024-03-31 DOI:10.1111/cid.13325

Sarmad Muayad Rasheed Al-Bakri, Antonio Magan-Fernandez, Pablo Galindo-Moreno, Francisco O'Valle, Natividad Martin-Morales, Miguel Padial-Molina, Francisco Mesa

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Abstract

Objective

The aim of this study was to detect and compare the tissular expression of neutrophil extracellular traps (NETs) in peri-implant and periodontal samples of patients with peri-implantitis, periodontitis, and controls.

Materials and Methods

An observational study was performed on patients with peri-implantitis, periodontitis, and controls. Peri-implant and/or periodontal clinical examinations were performed on each participant. Tissue samples were collected during tooth/implant extraction for clinical reasons. Electron microscopy analysis, Picro-Sirius red staining, immunohistochemical (CD15), and immunofluorescence (citrullinated H3 and myeloperoxidase) techniques were performed to detect NET-related structures and the degree of connective tissue destruction, between the study groups.

Results

Sixty-four patients were included in the study: 28 peri-implantitis, 26 periodontitis, and 10 controls, with a total of 51 implants, 26 periodontal teeth, and 10 control teeth. Neutrophil release of nuclear content was observed in transmission electron microscopy. Immunohistochemical analysis showed a greater CD15 expression in both peri-implantitis and periodontitis compared to controls (p < 0.001), and peri-implantitis presented lower levels of connective tissue and collagen compared to both periodontitis (p = 0.044; p < 0.001) and controls (p < 0.001). Immunofluorescence showed greater citH3 expression in peri-implantitis than the one found in both periodontitis (p = 0.003) and controls (p = 0.048).

Conclusions

A greater presence and involvement of neutrophils, as well as a greater connective tissue destruction were observed in cases of peri-implantitis. A higher expression of NET-related markers was found in mucosal samples of peri-implantitis compared to periodontitis and controls.

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查看原文本刊更多论文

检测和比较种植体周围炎、牙周炎和健康患者组织样本中的中性粒细胞胞外捕获物：试点研究。

研究目的本研究旨在检测和比较种植体周围炎、牙周炎患者和对照组的种植体周围和牙周样本中中性粒细胞胞外捕获器（NET）的组织表达：对种植体周围炎、牙周炎患者和对照组进行观察研究。对每位参与者进行了种植体周围和/或牙周临床检查。由于临床原因，在拔牙/拔种植体时采集了组织样本。通过电子显微镜分析、Picro-Sirius 红染色、免疫组织化学（CD15）和免疫荧光（瓜氨酸 H3 和髓过氧化物酶）技术检测研究组之间与 NET 相关的结构和结缔组织的破坏程度：研究共纳入64名患者：28名种植体周围炎患者、26名牙周炎患者和10名对照组患者，共计51颗种植体、26颗牙周炎牙齿和10颗对照组牙齿。透射电子显微镜观察到中性粒细胞释放核内容物。免疫组化分析表明，与对照组相比，种植体周围炎和牙周炎的 CD15 表达量更高（P 结论：种植体周围炎和牙周炎的 CD15 表达量更高：在种植体周围炎病例中观察到更多的中性粒细胞存在和参与，以及更严重的结缔组织破坏。与牙周炎和对照组相比，种植体周围炎的粘膜样本中NET相关标记物的表达量更高。

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来源期刊

Clinical Implant Dentistry and Related Research 医学-牙科与口腔外科

CiteScore

6.00

自引率

13.90%

发文量

103

审稿时长

4-8 weeks

期刊介绍： The goal of Clinical Implant Dentistry and Related Research is to advance the scientific and technical aspects relating to dental implants and related scientific subjects. Dissemination of new and evolving information related to dental implants and the related science is the primary goal of our journal. The range of topics covered by the journals will include but be not limited to: New scientific developments relating to bone Implant surfaces and their relationship to the surrounding tissues Computer aided implant designs Computer aided prosthetic designs Immediate implant loading Immediate implant placement Materials relating to bone induction and conduction New surgical methods relating to implant placement New materials and methods relating to implant restorations Methods for determining implant stability A primary focus of the journal is publication of evidenced based articles evaluating to new dental implants, techniques and multicenter studies evaluating these treatments. In addition basic science research relating to wound healing and osseointegration will be an important focus for the journal.