Establishment and validation of a 2D primary gill cell culture of the sevenband grouper (Hyporthodus septemfasciatus)

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2024-03-29 DOI:10.1016/j.jviromet.2024.114922

Rahul Rajendran , Rahul Krishnan , Myung-Joo Oh

引用次数: 0

Abstract

A 2D primary gill cell culture system of the sevenband grouper (Hyporthodus septemfasciatus) was established to validate the pathogenesis of nervous necrosis virus (NNV) as observed in previous studies. This system, developed using the double-seeded insert (DSI) technique, yielded confluent cell layers. Upon challenge with NNV in a setup containing both autoclaved salt water and L15 media in the apical compartment, viral replication akin to that anticipated based on previous studies was observed. Consequently, we advocate for the utilization of primary gill cell culture as a viable alternative to conventional methodologies for investigating host pathogen interactions.

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建立和验证七带石斑鱼（Hyporthodus septemfasciatus）的二维原代鳃细胞培养。

我们建立了七带石斑鱼（Hyporthodus septemfasciatus）的二维原代鳃细胞培养系统，以验证之前研究中观察到的神经坏死病毒（NNV）的致病机理。该系统采用双种子插入（DSI）技术开发，可产生汇合细胞层。在顶端区同时含有高压盐水和 L15 培养基的装置中，当受到 NNV 病毒挑战时，观察到的病毒复制与之前研究中预期的类似。因此，我们主张利用原代鳃细胞培养作为研究宿主病原体相互作用的传统方法的可行替代方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.

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