The first insight into Mycobacterium tuberculosis complex isolates in the lower northern region in Thailand.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-08-05 DOI:10.1093/trstmh/trae014

Janisara Rudeeaneksin, Supranee Bunchoo, Benjawan Phetsuksiri, Sopa Srisungngam, Ratchaneeporn Khummin, Jeewan Thapa, Chie Nakajima, Yasuhiko Suzuki

{"title":"The first insight into Mycobacterium tuberculosis complex isolates in the lower northern region in Thailand.","authors":"Janisara Rudeeaneksin, Supranee Bunchoo, Benjawan Phetsuksiri, Sopa Srisungngam, Ratchaneeporn Khummin, Jeewan Thapa, Chie Nakajima, Yasuhiko Suzuki","doi":"10.1093/trstmh/trae014","DOIUrl":null,"url":null,"abstract":"Background: Tuberculosis (TB) remains an important infectious disease and different genotypes have been reported. This study aimed to investigate the genetic diversity and molecular epidemiology of TB in the lower northern region of Thailand, where genotyping data are limited.Methods: A total of 159 Mycobacterium tuberculosis complex (MTBC) isolates from this region were genotyped by spoligotyping and the major spoligotypes were further subdivided by the mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) method.Results: Spoligotyping identified 34 types and classified them into 14 clusters. East African-Indian (EAI) groups were the most frequent (44.7%), followed by Beijing (36.5%), with a higher prevalence of drug resistance. By 15-loci MIRU-VNTR typing, the major groups of the Beijing and EAI2_NTB were further differentiated into 44 and 21 subtypes forming 9 and 5 subclusters with cluster rates of 0.26 and 0.44, respectively. The Hunter-Gaston Discriminatory Index among the Beijing and EAI2_NTB groups were 0.987 and 0.931, respectively, indicating high diversity.Conclusions: This is the first look at the MTBC genotypes in the lower northern region of Thailand, which could aid in understanding the distribution and potential spread of MTBC and Mycobacterium bovis in the target region to support TB control in Thailand.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/trstmh/trae014","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Tuberculosis (TB) remains an important infectious disease and different genotypes have been reported. This study aimed to investigate the genetic diversity and molecular epidemiology of TB in the lower northern region of Thailand, where genotyping data are limited.

Methods: A total of 159 Mycobacterium tuberculosis complex (MTBC) isolates from this region were genotyped by spoligotyping and the major spoligotypes were further subdivided by the mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) method.

Results: Spoligotyping identified 34 types and classified them into 14 clusters. East African-Indian (EAI) groups were the most frequent (44.7%), followed by Beijing (36.5%), with a higher prevalence of drug resistance. By 15-loci MIRU-VNTR typing, the major groups of the Beijing and EAI2_NTB were further differentiated into 44 and 21 subtypes forming 9 and 5 subclusters with cluster rates of 0.26 and 0.44, respectively. The Hunter-Gaston Discriminatory Index among the Beijing and EAI2_NTB groups were 0.987 and 0.931, respectively, indicating high diversity.

Conclusions: This is the first look at the MTBC genotypes in the lower northern region of Thailand, which could aid in understanding the distribution and potential spread of MTBC and Mycobacterium bovis in the target region to support TB control in Thailand.

查看原文本刊更多论文

首次深入了解泰国北部下游地区的结核分枝杆菌复合菌株。

背景：肺结核（TB）仍然是一种重要的传染性疾病，已有不同基因型的报道。本研究旨在调查基因分型数据有限的泰国北部下游地区结核病的遗传多样性和分子流行病学：方法：对来自该地区的 159 株结核分枝杆菌复合体（MTBC）分离株进行spoligotyping基因分型，并通过分枝杆菌穿插重复单元-变异数串联重复（MIRU-VNTR）方法对主要的spoligotypes进行进一步细分：Spoligotyping 鉴定出了 34 个类型，并将其分为 14 个群组。东非-印度（EAI）组最常见（44.7%），其次是北京组（36.5%），耐药率较高。通过 15 个基因组的 MIRU-VNTR 分型，北京和 EAI2_NTB 的主要群体被进一步区分为 44 个亚型和 21 个亚型，分别形成 9 个亚群和 5 个亚群，聚类率分别为 0.26 和 0.44。北京组和 EAI2_NTB 组之间的亨特-加斯顿鉴别指数分别为 0.987 和 0.931，表明多样性很高：这是对泰国北部下部地区 MTBC 基因型的首次研究，有助于了解 MTBC 和牛分枝杆菌在目标地区的分布和潜在传播情况，从而支持泰国的结核病控制工作。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.