Effector enrichment by Candidatus Liberibacter promotes Diaphorina citri feeding via Jasmonic acid pathway suppression

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2024-03-30 DOI:10.1002/ps.8107

Hao Liu, Bin Deng, Feng-Hao Chen, Jia-Qi Suo, Ge-Cheng Ouyang, Hui-Lin Lu, Da-Song Chen, Xiang Meng

{"title":"Effector enrichment by Candidatus Liberibacter promotes Diaphorina citri feeding via Jasmonic acid pathway suppression","authors":"Hao Liu, Bin Deng, Feng-Hao Chen, Jia-Qi Suo, Ge-Cheng Ouyang, Hui-Lin Lu, Da-Song Chen, Xiang Meng","doi":"10.1002/ps.8107","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> BACKGROUND</h3>\n \n <p>Citrus huanglongbing (HLB) is a devastating disease caused by <i>Candidatus</i> Liberibacter asiaticus (<i>C</i>Las) that affects the citrus industry. In nature, <i>C</i>Las relies primarily on <i>Diaphorina citri</i> Kuwayama as its vector for dissemination. After <i>D. citri</i> ingests <i>C</i>Las-infected citrus, the pathogen infiltrates the insect's body, where it thrives, reproduces, and exerts regulatory control over the growth and metabolism of <i>D. citri</i>. Previous studies have shown that <i>C</i>Las alters the composition of proteins in the saliva of <i>D. citri</i>, but the functions of these proteins remain largely unknown.</p>\n </section>\n \n <section>\n \n <h3> RESULTS</h3>\n \n <p>In this study, we detected two proteins (DcitSGP1 and DcitSGP3) with high expression levels in <i>C</i>Las-infected <i>D. citri</i>. Quantitative PCR and Western blotting analysis showed that the two proteins were highly expressed in the salivary glands and delivered into the host plant during feeding. Silencing the two genes significantly decreased the survival rate for <i>D. citri</i>, reduced phloem nutrition sucking and promoted jasmonic acid (JA) defenses in citrus. By contrast, after overexpressing the two genes in citrus, the expression levels of JA pathway-associated genes decreased.</p>\n </section>\n \n <section>\n \n <h3> CONCLUSION</h3>\n \n <p>Our results suggest that <i>C</i>Las can indirectly suppress the defenses of citrus and support feeding by <i>D. citri</i> via increasing the levels of effectors in the insect's saliva. This discovery facilitates further research into the interaction between insect vectors and pathogens. © 2024 Society of Chemical Industry.</p>\n </section>\n </div>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":null,"pages":null},"PeriodicalIF":3.8000,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pest Management Science","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/ps.8107","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

BACKGROUND

Citrus huanglongbing (HLB) is a devastating disease caused by Candidatus Liberibacter asiaticus (CLas) that affects the citrus industry. In nature, CLas relies primarily on Diaphorina citri Kuwayama as its vector for dissemination. After D. citri ingests CLas-infected citrus, the pathogen infiltrates the insect's body, where it thrives, reproduces, and exerts regulatory control over the growth and metabolism of D. citri. Previous studies have shown that CLas alters the composition of proteins in the saliva of D. citri, but the functions of these proteins remain largely unknown.

RESULTS

In this study, we detected two proteins (DcitSGP1 and DcitSGP3) with high expression levels in CLas-infected D. citri. Quantitative PCR and Western blotting analysis showed that the two proteins were highly expressed in the salivary glands and delivered into the host plant during feeding. Silencing the two genes significantly decreased the survival rate for D. citri, reduced phloem nutrition sucking and promoted jasmonic acid (JA) defenses in citrus. By contrast, after overexpressing the two genes in citrus, the expression levels of JA pathway-associated genes decreased.

CONCLUSION

Our results suggest that CLas can indirectly suppress the defenses of citrus and support feeding by D. citri via increasing the levels of effectors in the insect's saliva. This discovery facilitates further research into the interaction between insect vectors and pathogens. © 2024 Society of Chemical Industry.

Abstract Image

查看原文本刊更多论文

通过抑制茉莉酸途径，自由杆菌的作用因子富集可促进柠檬蝇的摄食。

背景：柑橘黄龙病（HLB）是由抗柑橘黄龙病菌（CLas）引起的一种毁灭性病害，影响柑橘产业。在自然界中，CLas 主要依靠桑山枸橘蝽作为传播媒介。枸橘蝽摄食受 CLas 感染的柑橘后，病原体就会渗入昆虫体内，在昆虫体内茁壮成长、繁殖，并对枸橘蝽的生长和新陈代谢进行调控。以前的研究表明，CLas 会改变柑橘褐飞虱唾液中蛋白质的组成，但这些蛋白质的功能在很大程度上仍然未知：qPCR 和 Western 印迹分析表明，这两种蛋白质在唾液腺中高表达，并在取食过程中被输送到寄主植物中。沉默这两个基因后，柑橘褐飞虱的存活率明显降低，韧皮部营养吸食减少，茉莉酸（JA）防御能力增强。相比之下，在柑橘中过表达这两个基因后，JA途径相关基因的表达水平下降：我们的研究结果表明，CLas 可以间接抑制柑橘的防御能力，并通过增加昆虫唾液中的效应物水平来支持柑橘褐飞虱的取食。这一发现有助于进一步研究昆虫媒介与病原体之间的相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.