Development of UV Method for Simultaneous Estimation of Imipramine and Ferulic Acid in Bulk and Developed Freeze-Dried Mixed Micelles

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-28 DOI:10.2174/0115734110286586240222100116

Naman Deep Raj, Dilpreet Singh

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引用次数: 0

Abstract

Introduction:: A simple, precise, and sensitive UV spectrophotometric method was developed to estimate Imipramine and Ferulic acid in bulk and polymeric micelles formulation. Moreover, imipramine and ferulic acid showed maximum absorbance at 237 nm and 216 nm. Method:: The method was validated for linearity, accuracy, precision, robustness, and ruggedness. The detector response for the imipramine and ferulic acid was linear over the selected range of 2 to 12 μg/ml with a correlation coefficient of 0.996 and 0.997. The accuracy was 99.4 and 101.02%. The precision (RSD) among six sample preparations was 0.68% and 0.85%. The method was validated as per the ICH guidelines. A polymeric micelle formulation was developed containing Vitamin E TPGS and F-127 as a surfactant and block co-polymer using different solvents. The optimized formulation containing 40 mg of F-127 and 30 mg of TPGS yielded the desired attributes of the optimized formulation. Result:: The optimized formulation was subjected to freeze-drying and yielded nanoparticulate size and excellent flowability. In vitro, the release of both drugs from the polymeric micelles was evaluated using dissolution, and multi-fold enhancement in release behavior was demonstrated compared to pure drugs. Both drugs were simultaneously detected successfully with accuracy and precision in bulk form and during in vitro analysis Conclusion:: The developed method can be adopted in routine analysis of imipramine and ferulic acid in bulk, and it involves relatively low-cost solvents with no complex extraction techniques.

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开发紫外法同时测定散装和已开发冻干混合胶束中的丙咪嗪和阿魏酸

简介：本研究采用紫外分光光度法测定了散装和聚合胶束制剂中的丙咪嗪和阿魏酸：建立了一种简单、精确和灵敏的紫外分光光度法，用于估算散装和聚合物胶束制剂中的丙咪嗪和阿魏酸含量。此外，丙咪嗪和阿魏酸在 237 nm 和 216 nm 处显示出最大吸光度。方法：对该方法的线性、准确度、精密度、稳健性和耐用性进行了验证。丙咪嗪和阿魏酸在 2 至 12 μg/ml 的选择范围内线性关系良好，相关系数分别为 0.996 和 0.997。准确度分别为 99.4% 和 101.02%。六种样品制剂的精密度（RSD）分别为 0.68% 和 0.85%。该方法按照 ICH 指南进行了验证。使用不同的溶剂开发了一种聚合物胶束配方，其中含有维生素 E TPGS 和作为表面活性剂和嵌段共聚物的 F-127。含有 40 毫克 F-127 和 30 毫克 TPGS 的优化配方达到了优化配方的预期属性。结果：：优化后的制剂经冷冻干燥后呈纳米颗粒状，流动性极佳。在体外，使用溶解法对两种药物从聚合物胶束中的释放情况进行了评估，结果表明，与纯药物相比，释放行为增强了数倍。在散装和体外分析过程中，成功地同时检测到了这两种药物：所开发的方法可用于散装亚胺培南和阿魏酸的常规分析，且所使用的溶剂成本相对较低，无需复杂的提取技术。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.