{"title":"Metabolomics Reveals the Impact of Zeolitic Imidazolate Framework-8 on Escherichia coli","authors":"","doi":"10.1134/s0003683824700029","DOIUrl":null,"url":null,"abstract":"<span> <h3>Abstract</h3> <p>The increasing use of metal organic frameworks can have many unintended consequences for ecosystems and the environment. However, knowledge of their toxicological behavior and mechanisms of action within the environmental microbial community is extremely limited. In this study, biological and metabolic responses of <em>Escherichia</em> <em>coli</em> to zeolitic imidazolate framework-8 (ZIF-8) were investigated. The results revealed that ZIF-8 could bind to and accumulate on the surface of <em>E. coli</em> cells. Low concentrations of ZIF-8 had little effects on the metabolism of <em>E. coli</em>, while exposure to high-dosage ZIF-8 led to perturbation of cellular functions, including changes of cell membrane permeability, leading to a shortage of energy supply. Moreover, the high-dosage ZIF-8 treatment induced stress-related biological pathways, such as increased metabolism of amino acids, sugars and sugar alcohols. All of these changes resulted in poor growth and low viability of <em>E. coli</em>. Taken together, the results provide detailed picture of the metabolic changes induced by ZIF-8 and are beneficial for assessing the toxicity of ZIF-8 and their further applications.</p> </span>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0003683824700029","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The increasing use of metal organic frameworks can have many unintended consequences for ecosystems and the environment. However, knowledge of their toxicological behavior and mechanisms of action within the environmental microbial community is extremely limited. In this study, biological and metabolic responses of Escherichiacoli to zeolitic imidazolate framework-8 (ZIF-8) were investigated. The results revealed that ZIF-8 could bind to and accumulate on the surface of E. coli cells. Low concentrations of ZIF-8 had little effects on the metabolism of E. coli, while exposure to high-dosage ZIF-8 led to perturbation of cellular functions, including changes of cell membrane permeability, leading to a shortage of energy supply. Moreover, the high-dosage ZIF-8 treatment induced stress-related biological pathways, such as increased metabolism of amino acids, sugars and sugar alcohols. All of these changes resulted in poor growth and low viability of E. coli. Taken together, the results provide detailed picture of the metabolic changes induced by ZIF-8 and are beneficial for assessing the toxicity of ZIF-8 and their further applications.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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