Alcohol and Caffeine Co-Administration Increased Acetylcholinesterase Activity and Inflammatory Cytokines in Sleep-Deprived Rats: Implications for Cognitive Decline and Depressive-Like Manifestations.

IF 1 Q4 CLINICAL NEUROLOGY
Sleep Science Pub Date : 2024-02-20 eCollection Date: 2024-03-01 DOI:10.1055/s-0043-1778013
Tarela Melish Elias Daubry, Bartholomew Chukwuebuka Nwogueze, Pere-Ebi Yabrade Toloyai, Emuesiri Goodies Moke
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Abstract

Sleep deprivation is a major health problem in modern society; it has been worsened by alcohol and caffeine intake to stay awake and improve bodily activities, an experience common among night-shift workers. For the present study, 50 adult male Wistar rats weighing between 150 g and 200 g were randomly selected and divided into 5 groups of 10 rats each (n = 10). Group 1 was the control group; group 2 was the group of sleep-deprived (SD) rats; group 3 was composed SD rats submitted to the administration of 20% alcohol; group 4 comprised SD rats submitted to the administration of 200 mg/kg of caffeine; and Group 5 was composed of SD rats who underwent the co-administration of 20% alcohol and 200 mg/kg of caffeine. At the end of 28 days, the animals were euthanized, and blood samples were collected for biochemical analysis. Memory, anxiety, social behavior and locomotive activity were assessed using the Y-maze, the elevated plus maze, the hole-board and three-chambered social approach tests, and the open field test. The plasma levels of the acetylcholinesterase (AChE) enzyme and inflammatory cytokines (interleukin 6 [IL-6], interleukin 10 [IL-10], and tumor necrosis factor beta, [TNF-β]) were also measured. Data was expressed as mean ± standard error of the mean [SEM] values, and the data were analyzed through analysis of variance (ANOVA) followed by the Tukey post hoc test, with significance set at p  < 0.05 . The results revealed that sleep deprivation, and the co-administration of alcohol and caffeine impair memory in rats. Sleep deprivation also caused a significant increase in anxiety and anxiety-related behavior, with decreased social interaction, in rats. Locomotive activity was improved in SD rats, especially in those to which alcohol was administered. Sleep deprivation significantly reduced acetylcholinesterase activity among SD rats and those to which alcohol was administered when compared with the controls. The plasma levels of IL-6, IL-10 and TNF-β were significantly increased in SD rats when compared with the controls. The administration of alcohol and caffeine separately, as well as their co-administration, significantly increased cytokine levels in rats.

酒精和咖啡因同时摄入会增加睡眠不足大鼠的乙酰胆碱酯酶活性和炎性细胞因子:认知能力下降和抑郁样表现的影响
睡眠不足是现代社会的一个主要健康问题;为保持清醒和改善身体活动而摄入酒精和咖啡因会加重睡眠不足,这在夜班工人中很常见。本研究随机挑选了 50 只体重在 150 克至 200 克之间的成年雄性 Wistar 大鼠,将其分为 5 组,每组 10 只(n = 10)。第1组为对照组;第2组为睡眠剥夺(SD)大鼠组;第3组为服用20%酒精的SD大鼠组;第4组为服用200毫克/千克咖啡因的SD大鼠组;第5组为同时服用20%酒精和200毫克/千克咖啡因的SD大鼠组。28 天后,动物被安乐死,并采集血液样本进行生化分析。用Y迷宫、高架加迷宫、洞板和三腔社会接近试验以及空地试验评估了动物的记忆、焦虑、社会行为和运动活动。此外,还测定了血浆中乙酰胆碱酯酶(AChE)和炎症细胞因子(白细胞介素6[IL-6]、白细胞介素10[IL-10]和肿瘤坏死因子β[TNF-β])的水平。数据以均数±均数标准误差[SEM]值表示,并通过方差分析(ANOVA)和Tukey事后检验对数据进行分析,显著性以p .结果表明,剥夺睡眠以及同时服用酒精和咖啡因会损害大鼠的记忆力。剥夺睡眠还会导致大鼠焦虑和焦虑相关行为显著增加,社会交往减少。SD大鼠的运动能力有所改善,尤其是那些服用了酒精的大鼠。与对照组相比,睡眠剥夺明显降低了自毁性大鼠和饮酒大鼠的乙酰胆碱酯酶活性。与对照组相比,SD 大鼠血浆中的 IL-6、IL-10 和 TNF-β 水平明显升高。分别给予酒精和咖啡因以及同时给予这两种物质都会显著增加大鼠的细胞因子水平。
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来源期刊
Sleep Science
Sleep Science CLINICAL NEUROLOGY-
CiteScore
2.50
自引率
12.50%
发文量
124
审稿时长
10 weeks
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