Genome-wide identification and expression analysis of TIFY genes under MeJA, cold and PEG-induced drought stress treatment in Dendrobium huoshanense

IF 3.4 3区生物学 Q1 PLANT SCIENCES

Physiology and Molecular Biology of Plants Pub Date : 2024-03-25 DOI:10.1007/s12298-024-01442-9

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引用次数: 0

Abstract

The TIFY family consists of plant-specific genes that regulates multiple plant functions, including developmental and defense responses. Here, we performed a comprehensive genomic analysis of TIFY genes in Dendrobium huoshanense. Our analysis encompassed their phylogenetic relationships, gene structures, chromosomal distributions, promoter regions, and patterns of collinearity. A total of 16 DhTIFY genes were identified, and classified into distinct clusters named JAZ, PPD, ZIM, and TIFY based on their phylogenetic relationship. These DhTIFYs exhibited an uneven distribution across 7 chromosomes. The expansion of the DhTIFY gene family appears to have been significantly influenced by whole-genome and segmental duplication events. The ratio of non-synonymous to synonymous substitutions (Ka/Ks) implies that the purifying selection has been predominant, maintaining a constrained functional diversification after duplication events. Gene structure analysis indicated that DhTIFYs exhibited significant structural variation, particularly in terms of gene organization and intron numbers. Moreover, numerous cis-acting elements related to hormone signaling, developmental processes, and stress responses were identified within the promoter regions. Subsequently, qRT-PCR experiments demonstrated that the expression of DhTIFYs is modulated in response to MeJA (Methyl jasmonate), cold, and drought treatment. Collectively, these results enhance our understanding of the functional dynamics of TIFY genes in D. huoshanense and may pinpoint potential candidates for detailed examination of the biological roles of TIFY genes.

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MeJA、低温和 PEG 诱导的干旱胁迫处理下铁皮石斛 TIFY 基因的全基因组鉴定和表达分析

摘要 TIFY家族由植物特异性基因组成，调控植物的多种功能，包括发育和防御反应。在此，我们对霍山石斛中的 TIFY 基因进行了全面的基因组分析。我们的分析涵盖了这些基因的系统发育关系、基因结构、染色体分布、启动子区域和共线性模式。共鉴定出 16 个 DhTIFY 基因，并根据其系统发育关系将其分为不同的群组，分别命名为 JAZ、PPD、ZIM 和 TIFY。这些 DhTIFY 在 7 条染色体上分布不均。DhTIFY 基因家族的扩展似乎受到了全基因组和区段复制事件的显著影响。非同义替换与同义替换之比（Ka/Ks）表明，纯化选择占主导地位，在复制事件后保持了受限的功能多样化。基因结构分析表明，DhTIFYs 在结构上表现出显著的变异，尤其是在基因组织和内含子数目方面。此外，在启动子区域还发现了许多与激素信号转导、发育过程和应激反应有关的顺式作用元件。随后的 qRT-PCR 实验表明，DhTIFYs 的表达受 MeJA（茉莉酸甲酯）、寒冷和干旱处理的影响。总之，这些结果加深了我们对霍山碱草中 TIFY 基因功能动态的了解，并可能为详细研究 TIFY 基因的生物学作用找到潜在的候选基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physiology and Molecular Biology of Plants PLANT SCIENCES-

CiteScore

7.10

自引率

0.00%

发文量

126

期刊介绍： Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.